黄芪百合颗粒对高原低氧模型小鼠肺自由基代谢及肺病理形态学改变的影响

发布时间：2019-05-27 01:48

【摘要】：目的:研究黄芪百合颗粒对高原低氧模型小鼠肺自由基代谢及其病理学形态改变的影响,探讨黄芪百合颗粒预防高原反应的效应机制。方法:将SPF级小鼠采用随机数字表法分为空白对照组,模型组,黄芪百合颗粒低、中、高剂量组。灌胃给药,每天1次,连续14 d。除对照组外,第15天起各组小鼠每天灌胃30 min后于低氧舱中模拟高海拔环境进行低氧暴露72 h。末次出舱称重后处死小鼠。比色法检测各组小鼠过氧化脂质降解产物丙二醛(malondialdehyde,MDA)的含量及总抗氧化能力(total antioxidantcapacity,T-AOC)、过氧化物歧化酶(super-oxide dismutase,SOD)的活性。HE染色法观察小鼠肺组织形态学改变情况。结果:与空白对照组比较,模型组小鼠体质量下降;肺脏、肾脏及心脏指数均升高;肺MDA的含量明显升高,SOD及T-AOC的活性明显下降;小鼠肺病理评分结果和肺炎性细胞数量均明显升高(P0.01)。与模型组比较,黄芪颗粒中、高剂量组小鼠有效体质量升高,肺脏、肾脏及心脏指数均降低;肺MDA含量下降,SOD及T-AOC活性均升高;小鼠肺病理评分结果和肺炎性细胞数量均下降(P0.05)。结论:黄芪百合颗粒通过调节自由基代谢明显增强了其抗高原肺水肿的作用。
[Abstract]:Aim: to study the effect of Huangqi lily granule on lung free radical metabolism and pathological morphology in mice with high altitude hypoxia, and to explore the mechanism of Huangqi lily granule in preventing high altitude reaction. Methods: SPF mice were randomly divided into blank control group, model group and low, middle and high dose groups. The drug was given intragastrically once a day for 14 days. Except the control group, the mice in each group were exposed to hypoxia for 72 hours at simulated high altitude for 30 min per day from the 15th day. The mice were killed the last time they were weighed out of the cabin. The content of malondialdehyde (malondialdehyde,MDA) and the total antioxidant capacity (total antioxidantcapacity,T-AOC) and SOD (super-oxide dismutase,) of lipid peroxide degradation products in each group were measured by colorimetric method. The activity of SOD). The morphological changes of lung tissue in mice were observed by HE staining. Results: compared with the blank control group, the body mass of the model group was decreased, the lung, kidney and heart index were increased, the content of MDA in the lung was significantly increased, and the activities of SOD and T-AOC were significantly decreased. The lung pathological score and the number of inflammatory cells in mice were significantly increased (P 0.01). Compared with the model group, in the high dose group, the effective body mass increased, the lung, kidney and heart index decreased, the MDA content in the lung decreased, and the activities of SOD and T-AOC increased in the high dose group. The results of lung pathological score and the number of inflammatory cells in mice decreased (P 0.05). Conclusion: Huangqi Lily granule can significantly enhance its anti-high altitude pulmonary edema by regulating free radical metabolism.
【作者单位】：甘肃中医药大学甘肃重大疾病分子医学与中医药防治研究省级重点实验室;敦煌医学与转化省部共建教育部重点实验室;
【基金】：2012甘肃省科技支撑计划资助项目(编号:120FKCA169) 陇原青年创新人才扶持计划资助项目(编号:2014-93-72)
【分类号】：R285.5;R-332

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