以气味作为相关环境线索的大鼠条件性位置偏好实验
发布时间：2019-05-28 08:23
【摘要】：实验目的：在条件性位置偏好实验中,为了最大程度区分两种环境,实验一般采用多线索条件刺激。但多线索条件刺激的注意之处是：环境刺激单独与非条件刺激形成的联系还是各种刺激组合成的实验情境与药物的奖赏作用形成的联系。因此,单一线索的条件性位置偏好实验可能有利于某些实验研究。本文以单一线索--气味作为相关环境线索进行条件性位置偏好实验,分析气味作为单一线索在大鼠吗啡条件性位置偏好实验中的作用。 实验方法：成年雄性SD大鼠,随机分为对应气味组、相反气味组和无气味组等三组,每组随机分为药物组(吗啡组)和对照组(生理盐水组)。实验系统由位置偏好行为测试箱和视频记录检测系统组成。位置偏好行为箱由两个大小相同的实验箱和一个中间过渡箱组成。两个实验箱的视觉和触觉均相同,两端分别放置滴有玫瑰原液或薄荷原液的小瓶。视频记录系统由安放在测试箱上方的摄像头和计算机软件系统组成,大鼠的行为数据由计算机软件记录和分析。实验分为三个阶段。第一阶段：适应阶段,即条件化前期。第1-3天,每天让大鼠在行为测试箱中自由活动15分钟(900秒),录像全过程,然后将大鼠分组放入各自房间3小时,每组的房间环境完全相同。第二阶段,条件化建立期。第4-11天,共8天。在玫瑰与薄荷中随机选择一种气味为阳性气味,对应的实验箱为阳性气味箱。每天给吗啡组大鼠腹腔注射吗啡(10mg/kg)或者同等剂量的生理盐水,放入实验箱45分钟。然后放入各自房间3小时,对应气味组的房间内给予当天在实验箱中相同气味；相反气味组的房间内给予与当天在实验箱中气味不同的另一种气味；无气味组则每天房间内不给予气味。生理盐水组大鼠每天注射等量生理盐水,其余操作与吗啡组相同。第三阶段,检测阶段。每天让大鼠在行为箱中自由活动15分钟,录像全过程。分析大鼠在两个实验箱中停留的时间。 数据分析方法：大鼠在CPP阳性气味箱停留时间以秒(s)为记录单位,采用SPSS11.5软件为统计工具进行数据分析。采用单因素方差分析和重复测量方差分析, P0.05为有统计学差异。 实验结果：条件化前期,采用单因素方差分析,各组大鼠在CPP阳性气味箱停留时间没有统计学差异(p0.05)；检测阶段,采用单因素方差分析和重复测量方差分析,药物处理与气味无交互效应(p＞0.05),各组大鼠在阳性气味箱停留时间没有统计学差异(p0.05)。 结论：气味作为单一线索未能建立大鼠吗啡条件性位置偏好,可能与气味具有挥发性,浓度难以控制等有关。
[Abstract]:Objective: in order to distinguish the two environments to the greatest extent, multi-clue conditional stimulation is usually used in conditional position preference experiment. However, the attention of multi-clue conditional stimulation is the relationship between environmental stimulation alone and non-conditional stimulation or the relationship between the experimental situation formed by the combination of various stimuli and the reward effect of drugs. Therefore, the conditional position preference experiment of a single clue may be beneficial to some experimental studies. In this paper, the conditioned position preference experiment was carried out with a single clue, odour as a related environmental clue, and the role of odor as a single clue in morphine conditioned position preference experiment in rats was analyzed. Methods: adult male SD rats were randomly divided into three groups: corresponding odor group, odor group and odorless group. Each group was randomly divided into drug group (morphine group) and control group (saline group). The experimental system is composed of position preference behavior test box and video recording detection system. The position preference behavior box consists of two experimental boxes of the same size and an intermediate transition box. The visual and tactile senses of the two experimental boxes were the same, with bottles dripped with rose solution or mint solution at both ends. The video recording system is composed of a camera and a computer software system placed above the test box, and the behavior data of rats are recorded and analyzed by computer software. The experiment is divided into three stages. The first stage: adaptation stage, that is, the early stage of conditionalization. On the 1st-3rd day, the rats were allowed to move freely in the behavior test box for 15 minutes (900 seconds) every day, and then the rats were grouped into their respective rooms for 3 hours, and the room environment of each group was exactly the same. The second stage, the conditional establishment period. 4-11 days, a total of 8 days. In rose and mint, a kind of smell was randomly selected as positive smell, and the corresponding experimental box was positive smell box. Morphine (10mg/kg) or the same dose of saline were injected intraperitoneally into morphine group every day and put in the experimental box for 45 minutes. Then put in their respective rooms for 3 hours, the room of the corresponding odor group was given the same smell in the experimental box on the same day, on the contrary, the room of the odor group was given another smell which was different from that in the experimental box on the same day. The odour free group did not give odour in the room every day. The rats in saline group were injected with the same amount of saline every day, and the other operations were the same as those in morphine group. The third stage, the detection stage. The rats were allowed to move freely in the behavior box for 15 minutes every day, and the whole process was videotaped. The time of rats staying in two experimental boxes was analyzed. Data analysis methods: the residence time of rats in CPP positive flavor box was recorded in second (s), and SPSS11.5 software was used as statistical tool to analyze the data. Using single factor variance analysis and repeated measurement variance analysis, P 0.05 was statistically significant. The results showed that there was no significant difference in the residence time of CPP positive flavor box in each group by single factor variance analysis in the early stage of conditionalization (p0.05). In the detection stage, single factor variance analysis and repeated measurement variance analysis were used. There was no interaction effect between drug treatment and smell (p > 0.05). There was no significant difference in the residence time of rats in positive flavor box (p0.05). Conclusion: odour as a single clue can not establish morphine conditioned position preference in rats, which may be related to odour volatility and difficult concentration control.
【学位授予单位】：昆明医学院
【学位级别】：硕士
【学位授予年份】：2011
【分类号】：R-332
[Abstract]:Objective: in order to distinguish the two environments to the greatest extent, multi-clue conditional stimulation is usually used in conditional position preference experiment. However, the attention of multi-clue conditional stimulation is the relationship between environmental stimulation alone and non-conditional stimulation or the relationship between the experimental situation formed by the combination of various stimuli and the reward effect of drugs. Therefore, the conditional position preference experiment of a single clue may be beneficial to some experimental studies. In this paper, the conditioned position preference experiment was carried out with a single clue, odour as a related environmental clue, and the role of odor as a single clue in morphine conditioned position preference experiment in rats was analyzed. Methods: adult male SD rats were randomly divided into three groups: corresponding odor group, odor group and odorless group. Each group was randomly divided into drug group (morphine group) and control group (saline group). The experimental system is composed of position preference behavior test box and video recording detection system. The position preference behavior box consists of two experimental boxes of the same size and an intermediate transition box. The visual and tactile senses of the two experimental boxes were the same, with bottles dripped with rose solution or mint solution at both ends. The video recording system is composed of a camera and a computer software system placed above the test box, and the behavior data of rats are recorded and analyzed by computer software. The experiment is divided into three stages. The first stage: adaptation stage, that is, the early stage of conditionalization. On the 1st-3rd day, the rats were allowed to move freely in the behavior test box for 15 minutes (900 seconds) every day, and then the rats were grouped into their respective rooms for 3 hours, and the room environment of each group was exactly the same. The second stage, the conditional establishment period. 4-11 days, a total of 8 days. In rose and mint, a kind of smell was randomly selected as positive smell, and the corresponding experimental box was positive smell box. Morphine (10mg/kg) or the same dose of saline were injected intraperitoneally into morphine group every day and put in the experimental box for 45 minutes. Then put in their respective rooms for 3 hours, the room of the corresponding odor group was given the same smell in the experimental box on the same day, on the contrary, the room of the odor group was given another smell which was different from that in the experimental box on the same day. The odour free group did not give odour in the room every day. The rats in saline group were injected with the same amount of saline every day, and the other operations were the same as those in morphine group. The third stage, the detection stage. The rats were allowed to move freely in the behavior box for 15 minutes every day, and the whole process was videotaped. The time of rats staying in two experimental boxes was analyzed. Data analysis methods: the residence time of rats in CPP positive flavor box was recorded in second (s), and SPSS11.5 software was used as statistical tool to analyze the data. Using single factor variance analysis and repeated measurement variance analysis, P 0.05 was statistically significant. The results showed that there was no significant difference in the residence time of CPP positive flavor box in each group by single factor variance analysis in the early stage of conditionalization (p0.05). In the detection stage, single factor variance analysis and repeated measurement variance analysis were used. There was no interaction effect between drug treatment and smell (p > 0.05). There was no significant difference in the residence time of rats in positive flavor box (p0.05). Conclusion: odour as a single clue can not establish morphine conditioned position preference in rats, which may be related to odour volatility and difficult concentration control.
【学位授予单位】：昆明医学院
【学位级别】：硕士
【学位授予年份】：2011
【分类号】：R-332
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