大鼠虚热证模型的建立与评价

发布时间：2019-05-30 05:56

【摘要】：目的:对热性中药致虚热证模型的复制和评价进行探究。方法:使用不同剂量热性中药附子、干姜、肉桂灌胃大鼠。每天观察大鼠的活动状态、毛发光泽、爪色、二便等一般状况,每天定时测定大鼠的肛温、粪便量、尿液量、饮水量、饮食量,并于体液消耗量出现明显差异时采集血液和相关组织器官,测定血清三碘甲状腺原氨酸(T3),甲状腺素(T4),血浆环磷酸腺苷(cAMP)及环磷酸鸟苷(cGMP)含量的变化和相关脏器指数。结果:与空白组比较,各模型组大鼠饮水量、体液消耗量大多数升高,动物出现虚热证的证候;且血清中T4含量呈增高趋势,T3/T4降低。模型低剂量组和模型中剂量组的cAMP和cGMP含量与空白组比较大多数显著升高,而模型高剂量组含量则有所降低。结论:灌胃热性中药可以复制出虚热证模型,体液消耗量的增加对虚热证模型复制有一定的指导意义。
[Abstract]:Objective: to explore the replication and evaluation of heat deficiency syndrome model caused by heat traditional Chinese medicine (TCM). Methods: different doses of heat traditional Chinese medicine aconite, dried ginger and cinnamon were used intragastrically in rats. The activity, hair luster, claw color and second stool of the rats were observed every day. The anal temperature, fecal volume, urine volume, water consumption and food intake of the rats were measured regularly every day. Blood and related tissues and organs were collected when there was significant difference in body fluid consumption, and serum triiodothyronine (T3) and thyroxine (T4) were measured. Changes of plasma cyclic adenosine monophosphate (cAMP) and cyclic guanosine monophosphate (cGMP) content and related organ index. Results: compared with the blank group, most of the rats in each model group had higher drinking water and body fluid consumption, and the syndrome of deficiency heat syndrome appeared, and the content of T 4 in serum was increased and T3/T4 decreased. The contents of cAMP and cGMP in the low dose group and the middle dose group were significantly higher than those in the blank group, while those in the high dose group were lower than those in the blank group. Conclusion: traditional Chinese medicine can replicate the model of deficiency heat syndrome, and the increase of body fluid consumption has certain guiding significance for the replication of deficiency heat syndrome model.
【作者单位】：江西中医药大学;
【基金】：国家自然科学基金项目(81260642) 江西省研究生创新专项(YC2015-B066)
【分类号】：R285.5;R-332

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