乳腺癌气虚血瘀证小鼠模型的建立及益气活血法的干预研究
[Abstract]:Objective To establish a 4-T1 mouse model of breast cancer with Qi-deficiency and blood stasis syndrome, and to treat the tumor microenvironment by using the traditional Chinese medicine in the condition of qi-deficiency and blood stasis. Methods Forty-eight female BALB/ c mice were randomly divided into 6 groups: the blank control group, the pure tumor-bearing group, the Qi-deficiency and blood-stasis syndrome-bearing model group (compound model group), the low-dose group of the composite model, the dose group in the composite model, and the high-dose group of the compound model. The mice were injected with 4T1 single cell suspension, and the compound model group was injected with reserpine on the basis of the simple tumor-bearing group. From the 15th day of the experiment, the low-dose group, middle-dose group and high-dose group of the compound model were given the corresponding dose of the traditional Chinese medicine (Sijunzi Decoction and Xuefu Zhuyu Decoction), once a day, for 28d, respectively. The expression of matrix metalloproteinase-2 (MMP2) and extracellular signal-regulated kinase (p-ERK1/2) was determined by Western blot. Results The combined model group, the low-dose group of the compound model, the dose group in the compound model, the high-dose group of the compound model had the symptoms of Qi deficiency and blood stasis, and the quantitative score of the low-dose group was lower than that of the compound model group at the 28th day (P0.05), and the score of the middle-dose group was lower than that of the compound model group (P0.05). The blood flow of the vein in the blank control group was greater than that of the other groups (P0.05). The combined model group was less than that of the simple tumor-bearing group (P0.05). The intervention group of the traditional Chinese medicine group was higher than that of the compound model group (P 0.01 or P0.05). The expression of MMP-2 in the compound model group was higher than that of the simple tumor-bearing group (P0.05). In the low-dose group, the expression of p-ERK1/2 in the middle-dose group was lower than that of the compound model group (P0.05 or P0.01). Conclusion The model of breast cancer with Qi-deficiency and blood stasis syndrome established by injection of 4T1 single cell suspension in combination with the injection of reserpine was stable and reliable, and it was in accordance with the characteristics of TCM syndrome. The expression of MMP-2 and p-ERK1/2 in the tumor tissue of mice with Qi-deficiency and blood stasis syndrome was up-regulated, and the expression of MMP-2 and p-ERK1/2 was down-regulated in the condition of qi-deficiency and blood stasis, and the growth of the tumor was inhibited.
【作者单位】: 浙江中医药大学附属第二医院全科医学科;浙江中医药大学附属第一医院肿瘤科;浙江中医药大学;
【基金】:浙江省自然科学基金项目(No.Y13H290038) 浙江省中医药科学研究基金计划项目(No.2012ZA049)~~
【分类号】:R285.5;R-332
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