STAT3与STAT5在小鼠卵巢组织发育过程中的定位及表达变化

发布时间：2019-06-10 02:09

【摘要】：目的探讨STAT3与STAT5在小鼠卵巢组织发育过程中的定位及表达变化。方法收集胚胎13.5 d及出生后1、7、14、21、28、106 d的C57BL/6J小鼠卵巢组织,用免疫荧光染色法检测各级卵泡中STAT3和STAT5蛋白表达,RT-PCR技术检测小鼠卵巢组织发育过程中STAT3、STAT5 mRNA表达。结果 STAT3在原始卵泡、初级卵泡卵母细胞的细胞质和细胞核中高表达,在次级卵泡和窦状卵泡卵母细胞的细胞核中低表达,在其他细胞如颗粒细胞中不表达。出生后1、7 d STAT3 mRNA相对表达量与其他时间点比较,P均0.05。STAT5在原始卵泡、初级卵泡卵母细胞的细胞核中高表达,在初级卵泡、次级卵泡的颗粒细胞及部分次级卵泡卵母细胞的核仁区低表达。出生后14 d STAT5a mRNA相对表达量与胚胎13.5 d及出后生1、7 d比较,P均0.05。出生后106 d STAT5b mRNA相对表达量与其他时间点比较,P均0.05。结论 STAT3与STAT5在小鼠卵巢组织发育过程中均有不同程度的表达,且其定位与表达随着小鼠卵泡的发育而变化。
[Abstract]:Objective to investigate the localization and expression of STAT3 and STAT5 during the development of mouse ovarian tissue. Methods the ovarian tissues of C57BL/6J mice were collected from 13. 5 days after embryo and 1, 7, 14, 21, 28106 days after birth. The expression of STAT3 and STAT5 protein in follicles of all levels was detected by immunofluorescence staining, and the expression of STAT3,STAT5 mRNA during ovarian tissue development was detected by RT-PCR. Results STAT3 was highly expressed in the cytoplasm and nucleus of primordial follicles and primary follicles, but low in the nucleus of secondary follicles and antral oocytes, but not in other cells such as granulosa cells. Compared with other time points, the relative expression of STAT3 mRNA on the 1st and 7th day after birth was highly expressed in the nucleus of primordial follicles and primary follicles, and in the primary follicles. The granulosa cells of secondary follicles and some secondary follicular oocytes were underexpressed in nucleolus. The relative expression of STAT5a mRNA on the 14th day after birth was significantly higher than that on the 13th day after birth and on the 1st day after birth, and P was 0.05 on the 7th day after birth. Compared with other time points, the relative expression of STAT5b mRNA on the 106th day after birth was 0.05. Conclusion STAT3 and STAT5 are expressed in different degrees during the development of mouse ovarian tissue, and their localization and expression change with the development of mouse follicles.
【作者单位】：南京中医药大学附属中西医结合医院·江苏省中西医结合医院;南京市妇幼保健院·南京医科大学附属妇产医院;
【基金】：国家自然科学基金资助项目(81602285)
【分类号】：R339.22

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