中药柴贝止痫汤对难治性癫痫大鼠模型行为学及P-糖蛋白和MDR1表达的影响
发布时间：2019-06-17 08:28
【摘要】：目的:观察柴贝止痫汤、卡马西平、柴贝止痫汤与卡马西平联用3种不同干预对难治性癫痫大鼠模型行为学及P-糖蛋白(P-gp)和MDR1表达的影响。方法:采用侧脑室注射海人酸法制作癫痫持续状态后慢性难治性癫痫大鼠模型,分为模型组、西药组、中药组、中西药组、正常组、假手术组6组。给药时间3个月。以Western Blot及实时荧光定量PCR法检测治疗1、3个月海马P-gp及MDR1a/1b mRNA水平。结果:海马中P-gp、MDR1a/1b mRNA含量,模型组高于假手术组,在治疗1个月差异具有统计学意义(P0.01,P0.05);西药组高于模型组,差异不具统计学意义;中药组、中西药组低于模型组,其中P-gp含量在治疗1月时差异具有统计学意义(P0.05);中西药组低于西药组,差异具有统计学意义(P0.05)。结论:柴贝止痫汤能够抑制难治性癫痫P-gp表达,这可能通过对MDR1a/1b mRNA转录水平的影响实现。
[Abstract]:Aim: to observe the effects of Chaibei Zhixian decoction, carbamazepine, Chaibei Zhixian decoction and carbamazepine on behavior and expression of P-glycoprotein (P-gp) and MDR1 in refractory epileptic rats. Methods: the rat model of chronic refractory seizures after persistent status was established by intracerebroventricular injection of kainic acid. The rats were divided into 6 groups: model group, western medicine group, Chinese and western medicine group, normal group and false operation group. The drug was given for 3 months. The levels of P-gp and MDR1a/1b mRNA in hippocampus were measured by Western Blot and real-time fluorescence quantitative PCR at 1 and 3 months after treatment. Results: the content of P 鈮，
本文编号：2500852
[Abstract]:Aim: to observe the effects of Chaibei Zhixian decoction, carbamazepine, Chaibei Zhixian decoction and carbamazepine on behavior and expression of P-glycoprotein (P-gp) and MDR1 in refractory epileptic rats. Methods: the rat model of chronic refractory seizures after persistent status was established by intracerebroventricular injection of kainic acid. The rats were divided into 6 groups: model group, western medicine group, Chinese and western medicine group, normal group and false operation group. The drug was given for 3 months. The levels of P-gp and MDR1a/1b mRNA in hippocampus were measured by Western Blot and real-time fluorescence quantitative PCR at 1 and 3 months after treatment. Results: the content of P 鈮，
本文编号：2500852
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