金黄色葡萄球菌细胞内生物大分子含量变化的光镊拉曼光谱研究

发布时间：2019-06-20 01:20

【摘要】：【目的】利用光镊拉曼光谱技术(Laser Tweezers Raman Spectroscopy,LTRS)研究金黄色葡萄球菌(Staphylococcus aureus)细胞内主要生物大分子含量的动态变化,探寻监测微生物代谢状态变化的有效工具。【方法】收集分批培养过程不同时间点金黄色葡萄球菌细胞的拉曼光谱,观察其特征峰依赖时间的变化特点。【结果】归属于核酸的特征峰,如667cm~(-1)峰,724cm~(-1)峰,781cm~(-1)峰,809cm~(-1)峰和1 574cm~(-1)峰在整个分批生长阶段持续降低,在对数生长期下降幅度尤其剧烈,反映了对数生长期细胞分裂快速,DNA复制活跃;与蛋白质相关的特征峰,如850cm~(-1)峰,1 001cm~(-1)峰和1 662cm~(-1)峰,在对数生长期显著增强,而到稳定期峰强度基本维持恒定。【结论】光镊拉曼光谱技术能实时监测生物样品内生物大分子的含量,是单细胞水平上研究微生物代谢状态变化的有效工具。
[Abstract]:[objective] to study the dynamic changes of main biological macromolecules in Staphylococcus aureus (Staphylococcus aureus) cells by optical tweezers Raman spectroscopy (Laser Tweezers Raman Spectroscopy,LTRS), and to explore an effective tool to monitor the changes of microbial metabolism. [methods] Raman spectra of Staphylococcus aureus cells at different time points during batch culture were collected. The variation of characteristic peak dependent time was observed. [results] the characteristic peaks attributed to nucleic acid, such as 667cm1 peak, 724cm1 peak, 781cm1 peak, 809cm1 peak and 1574 cm1 peak, decreased continuously in the whole batch growth stage, especially in logarithmic growth period, which reflected the rapid cell division and active DNA replication in logarithmic growth period. The characteristic peaks related to protein, such as 850 cm ~ (- 1) peak, 1 001 cm ~ (- 1) peak and 1 662 cm ~ (- 1) peak, increased significantly during logarithmic growth period, but remained constant at stable stage. [conclusion] Optical tweezers Raman spectroscopy can monitor the content of biological macromolecules in biological samples in real time, and it is an effective tool to study the changes of microbial metabolism at single cell level.
【作者单位】：广西科学院生物物理实验室;百色学院物理与电信工程系;
【基金】：广西自然科学基金项目(2014GXNSFAA118193) 广西科学院基本科研业务费项目(15YJ22SL08)资助
【分类号】：R378.11;O657.37

【相似文献】