头花蓼对幽门螺杆菌粘附定植的影响

发布时间：2019-06-26 22:29

【摘要】：目的观察头花蓼水提物对幽门螺杆菌(Helicobacter pylori,H.pylori)粘附定植的影响。方法采用尿素酶活性比色法定量检测头花蓼水提物作用前后幽门螺杆菌尿素酶活性的影响;运用半固体布氏琼脂平板法检测不同浓度头花蓼对幽门螺杆菌鞭毛运动的影响;利用细胞爬片革兰染色观察不同浓度头花蓼作用后幽门螺杆菌在GES-1细胞表面粘附的变化并计算各组抑制率;采用Real-time PCR技术检测头花蓼作用前后相关基因ureB、ureE、ureF、flaA、flaB、babA、alpA、alpB的mRNA转录水平。结果头花蓼水提物对幽门螺杆菌尿素酶活性具有抑制作用,抑制率为44.90±0.289。头花蓼浓度为1/2MIC、1/4MIC、1/8MIC分别作用后幽门螺杆菌菌膜晕圈直径显著小于对照组,分别为(5.67±0.55)mm,(8.5±0.50)mm,(11.67±1.53)mm,差异有统计学意义(P0.05);不同浓度头花蓼水提物均能抑制细菌粘附胃上皮细胞,各浓度组与对照组比较,差异有统计学意义(P0.05)。头花蓼作用后,与对照组比较,下调了尿素酶结构基因ureB,尿素酶活性基因ureE、ureF、鞭毛相关基因flaB、flaA以及粘附素基因babA、alpA、alpB mRNA的转录水平,差异均具有统计学意义(P0.05)。结论头花蓼对幽门螺杆菌具有明显的抑制作用,是通过抑制幽门螺杆菌尿素酶活性,减缓幽门螺杆菌的鞭毛动力,影响幽门螺杆菌对胃粘膜上皮细胞的粘附等方面发挥其抑菌作用。
[Abstract]:Objective to observe the effect of water extract of Polygonum capitatum on adhesion and colonization of Helicobacter pylori,H.pylori. Methods the effect of urease activity before and after the water extract of Polygonum capitatum was detected quantitatively, the effect of different concentrations of Polygonum capitatum on the flagella movement of HP was detected by semi-solid Brinell Agar plate method, and the adhesion of HP to the surface of GES-1 cells was observed by cell climbing Gram staining and the inhibition rate of each group was calculated. The mRNA transcription level of ureB,ureE,ureF,flaA,flaB,babA,alpA,alpB gene before and after the action of Polygonum capitatum was detected by Real-time PCR. Results the water extract of Polygonum capitatum had inhibitory effect on urease activity of HP, and the inhibitory rate was 44.90 卤0.289. The concentration of Polygonum capitatum was 1) mm, (, 1 Mc, and the diameter of membrane halo of HP was significantly smaller than that of the control group, which was (5.67 卤0.55) mm, (卤0.50) mm, (11.67 卤1.53) mm, respectively (P 0.05), and the diameter of the membrane halo was significantly lower than that of the control group (P < 0.05), and there was significant difference between the two groups (P 0.05). Different concentrations of water extract of Polygonum capitatum could inhibit bacterial adhesion to gastric epithelial cells, and there was significant difference between each concentration group and the control group (P 0.05). After the action of Polygonum capitatum, compared with the control group, the transcription level of urease structure gene ureB, urease activity gene ureE,ureF, flagella related gene flaB,flaA and adhesin gene babA,alpA,alpB mRNA was down-regulated, the difference was statistically significant (P 0.05). Conclusion Polygonum polygonum has obvious inhibitory effect on HP, which plays an important role in inhibiting the activity of urease, slowing down the flagella dynamics of HP and affecting the adhesion of HP to gastric epithelial cells.
【作者单位】：贵州医科大学临床检验教研室;贵州医科大学附属医院检验科;
【基金】：贵州省科技合作计划基金(No.黔科合LH(2015)7417号) 贵州省卫生计生委科学技术基金(No.gzwjkj2015-1-003) 2014年地方高校国家级大学生创新创业训练计划项目(No.201410660014) 2014年贵州省大学生创新创业训练计划项目(No.201410660014)联合资助~~
【分类号】：R378

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