铜绿假单胞菌分泌物对中性粒细胞趋化运动的抑制功能

发布时间：2019-06-28 09:35

【摘要】：目的研究铜绿假单胞菌分泌物对中性粒细胞趋化功能的影响。方法用percoll密度梯度离心法分离小鼠骨髓中性粒细胞并用FACS检测其纯度;铜绿假单胞菌PAO1静置培养5 d后涡旋并离心收集其上清;通过TAXIScan-FL检测被膜上清PAO1 d5(PAO1菌株静置培养第5天)的趋化作用;不同浓度的f MLP所引起的中性粒细胞趋化运动及PAO1 d5上清稀释50倍后的趋化作用;PAO1 d5上清对f MLP所引起的中性粒细胞趋化运动的影响。结果分离的小鼠骨髓中性粒细胞纯度大于90%;TAXIScan-FL检测被膜上清有趋化作用,但是速度及方向上与f MLP及LTB4有明显差别(P0.001);高浓度的f MLP对中性粒细胞的趋化作用减弱,PAO1 d5稀释50倍后趋化作用消失;PAO1 d5上清可以抑制f MLP所引起的中性粒细胞趋化运动。结论 PAO1d5被膜上清中存在抑制中性粒细胞趋化的物质。
[Abstract]:Objective to study the effect of Pseudomonas aeruginosa exudates on neutrophil chemotactic function. Methods the bone marrow neutrophils of mice were isolated by percoll density gradient centrifugation and their purity was detected by FACS. After 5 days of static culture of Pseudomonas aeruginosa PAO1, the supernatant was collected by centrifugation; the chemotactic effect of PAO1 d5 (PAO1 strain) was detected by TAXIScan-FL, and the chemotactic activity of neutrophils induced by different concentrations of f MLP and the chemotactic effect of PAO1 d 5 supernatant diluted 50 times. Effect of PAO1 d 5 on neutrophil chemotaxis induced by f MLP. Results the purity of the isolated mouse bone marrow neutrophils was more than 90%. The chemotactic effect of the membrane was detected, but it was significantly different from that of f MLP and LTB4 in speed and direction (P0.001). The chemotactic effect of high concentration of f MLP on neutrophils was weakened, and the chemotactic effect of PAO1 d 5 was disappeared after dilution 50 times. PAO1 d 5 could inhibit the chemotaxis of neutrophils induced by f MLP. Conclusion there is an inhibitor of neutrophil chemotaxis in PAO1d5 capsule.
【作者单位】：血液病医院中国科学院南海海洋研究所
【基金】：国家自然科学基金(31271484,31471116)
【分类号】：R392

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