小肠结肠炎耶尔森菌在达乌尔黄鼠鼠疫疫源地分布及特征研究

发布时间：2019-06-29 08:11

【摘要】：目的了解小肠结肠炎耶尔森菌在达乌尔黄鼠鼠疫疫源地动物中的种间带菌分布情况及其特征,为我国小肠结肠炎耶尔森菌的研究提供科学的参考数据。方法在达乌尔黄鼠鼠疫疫源地内选择牧区、半农半牧区和农区3个不同生态环境,分别采集鼠类、家畜家禽动物的粪便、舌根、咽拭子及肠道内容物等样本,进行小肠结肠炎耶尔森菌的分离、鉴定和毒力基因检测。结果共检测各类样本3 260份,检出小肠结肠炎耶尔森菌65株,总检出率为1.99%;猪的带菌率最高,其携带O∶3/3、O∶5/1A、O∶4/4 3个血清生物型;其中O∶3/3型致病性菌株携带ail、ystA、yadA、VirF、rfbc毒力基因;且在牧区、半农半牧区、农区的样本均可检出阳性菌,以农区最高,检出率为9.95%。结论小肠结肠炎耶尔森菌在内蒙古达乌尔黄鼠鼠疫疫源地中分布较广,以农区为重;猪是致病性小肠结肠炎耶尔森菌的主要携带者。
[Abstract]:Objective to investigate the distribution and characteristics of Yersinia enterocolitis in plague foci of Daour gerbils, and to provide scientific reference data for the study of Yersinia enterocolitis in China. Methods three different ecological environments, including pastoral area, semi-agricultural and semi-pastoral area and agricultural area, were selected from Daour yellow rat plague focus. the feces, tongue root, throat swabs and intestinal contents of rodent, livestock and poultry were collected to isolate, identify and detect virulence genes of Yelsen enterocolitis. Results A total of 3260 samples were detected, 65 strains of Yelsen enterocolitis were detected, the total detection rate was 1.99%, the carrying rate of pigs was the highest, carrying O _ 3 鈮，

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