青海省251株结核分枝杆菌Spoligotyping基因型与4种一线药物耐药表型的研究

发布时间：2019-08-26 12:16

【摘要】：目的研究青海结核分枝杆菌对4种一线抗结核药物耐药状况及与Spoligotyping基因型的关系,为结核病有效预防提供依据。方法采用常规比例法对分离的251株结核分枝杆菌进行异烟肼(INH)、利福平(RFP)、链霉素(SM)和乙胺丁醇(EMB)等4种一线抗结核药物的药敏试验,并对菌株进行Spoligotyping分型,对药敏试验和基因分型结果进行综合分析。结果 251株结核分枝杆菌中,总耐药率为56.2%;对INH、RFP、SM和EMB的耐药率分别为43.0%(108/251)、37.1%(93/251)、39.0%(98/251)和27.9%(70/251);耐多药率为31.5%(79/251)。所有菌株经Spoligotyping分型,分为北京基因型185株(73.7%)与非北京型菌株66株(26.3%)。未发现北京型与非北京型结核菌株与耐药存在统计学关联。结论青海流行的结核分枝杆菌耐药率及多耐药率较高,Spoligotyping分型显示北京基因型为主要流行型。
[Abstract]:Objective to study the resistance of Mycobacterium tuberculosis to four first-line antituberculosis drugs and its relationship with Spoligotyping genotype, so as to provide evidence for effective prevention of tuberculosis. Methods the drug sensitivity test of isoniazid (INH), rifampicin (RFP), streptomycin (SM) and ethambutol (EMB) was carried out on 251 strains of Mycobacterium tuberculosis by conventional proportional method. The strains were typed by Spoligotyping, and the results of drug sensitivity test and genotyping were analyzed comprehensively. Results among 251 strains of Mycobacterium tuberculosis, the total drug resistance rate was 56.2%, the resistance rate to INH,RFP,SM and EMB was 43.0% (108 鈮，

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