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载银二氧化钛抗菌涂层气管插管导管的研制和基础研究

发布时间:2018-01-03 06:04

  本文关键词:载银二氧化钛抗菌涂层气管插管导管的研制和基础研究 出处:《浙江中医药大学》2014年博士论文 论文类型:学位论文


  更多相关文章: 气管插管导管 抗菌涂层 载银二氧化钛 表征 抗菌性能 不良反应


【摘要】:目的研发一种能预防气管插管机械通气引发的呼吸机相关性肺炎的气管插管导管。 方法以医用聚乙烯气管插管导管为基材、载银二氧化钛为抗菌剂、TEOS为硅源、KH570和MTES为有机相前驱体,通过溶胶-凝胶法制备TEOS/KH570/MTES/Ag-TiO2抗菌有机硅溶胶,将聚乙烯气管插管导管表面活化处理,通过浸渍涂布法制备TEOS/KH570/MTES/Ag-TiO2抗菌涂层气管插管导管。进而研究分析抗菌涂层的表征:通过溶胶稳定性测试、涂层硬度和附着力测试、涂层红外光谱分析、涂层表面形貌分析、涂层透光性检测和ICP-MS测试,以确定载银二氧化钛抗菌涂层的成分配比;选取标准铜绿假单胞菌、大肠埃希菌、金黄色葡萄球菌作为实验观察菌株,通过浸渍菌液培养法检测涂层导管抗菌性能;通过口腔黏膜刺激试验、热原检查、溶血试验、急性全身毒性试验和体外细胞毒性试验,以明确其是否存在生物不良反应,确定抗菌涂层导管的生物相容性和生物安全性。 结果TEOS/KH570/MTES/Ag-TiO2=15/6/1/≤1.0抗菌溶胶在聚乙烯气管插管导管表面附着力(0级)、表面硬度(5H)、溶胶稳定不沉淀,说明抗菌溶胶涂层与聚乙烯气管插管导管表面紧密附着,既保证了导管的柔韧性,又保证涂层在压折后不易断裂,而且溶胶涂层均匀稳定;涂层红外光谱分析显示TEOS/KH570/MTES/Ag-TiO2(15/6/1/1.0)抗菌溶胶交联结合稳定,保证涂层成分形成一体,不易掉落;涂层表面形貌分析显示涂层表面非常均匀平整,无小颗粒和相分离现象,涂层表面网络结构致密,稳定无分离,保证了涂层导管的光滑;涂层透光性检测显示平均透过率90%,且具有增透效果;ICP-MS测试,银离子析出几乎可以忽略不计,说明抗菌涂层管具有良好的抗菌性能和稳定性。抗菌试验显示载银二氧化钛抗菌涂层气管插管导管具有明确的抗菌作用,能杀灭铜绿假单胞菌、金黄色葡萄球菌、大肠埃希菌,在不同的抗菌剂浓度时杀菌强度不同。抗菌涂层气管插管导管涂层载银二氧化钛含量越高,则抗菌性能越强,达到1%浓度后,则处于饱和杀菌状态,可几乎完全杀灭所有细菌,说明1%载银二氧化钛抗菌涂层气管插管导管是有效抗菌的最低浓度。口腔黏膜刺激试验显示1%载银二氧化钛抗菌涂层气管插管导管浸提液对金黄地鼠口腔粘膜未出现刺激反应;热原检查显示涂层管浸提液3h内的最高升温分别为0℃,0℃,0.03℃,总升温为0.03℃;溶血试验显示涂层管的吸光度分别为:0.016、0.014、0.021,平均值为0.0170,计算得溶血率为0.7512%;急性全身毒性试验显示涂层管浸提液在整个试验过程均无动物死亡,且未见异常反应,体外细胞毒性试验细胞形态正常,贴壁生长良好,胞浆内有离散颗粒,无细胞溶解,评为无毒,判定分级为0级,相对细胞增殖率均大于100%。 结论以TEOS/KH570/MTES/Ag-Ti02=15/6/1≤1.0比例和1%浓度载银二氧化钛抗菌剂制备的抗菌涂层气管插管导管表征优良,抗菌性能优越,满意的生物相容性和安全性,可以作为预防VAP的临床前试验候选导管。
[Abstract]:Objective to develop a tracheal intubation catheter that can prevent ventilator-associated pneumonia caused by endotracheal intubation.
Methods medical polyethylene endotracheal intubation catheter as the substrate, silver loaded titanium dioxide as antibacterial agent, TEOS as silicon source, KH570 and MTES as organic precursor, prepared by sol-gel method TEOS/KH570/MTES/Ag-TiO2 antibacterial silicone sol, polyethylene endotracheal tube surface activation, by dip coating prepared by TEOS/KH570/MTES/Ag-TiO2 antibacterial coating the endotracheal tube. Further characterization of the antibacterial coating by sol stability test, hardness test and adhesion test, infrared spectrum analysis analysis of coating, coating surface morphology, coating light detection and the ICP-MS test to determine the composition of silver doped titania antibacterial coating; the criteria for the selection of Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus as observed by dipping strains, bacterial liquid antibacterial properties were detected through buccal adhesive coating catheter; Membrane stimulation test, pyrogen test, hemolysis test, acute systemic toxicity test and in vitro cytotoxicity test were performed to determine whether there were any adverse reactions and determine the biocompatibility and biosafety of the antibacterial coating catheter.
The results of TEOS/KH570/MTES/Ag-TiO2=15/6/1/ is less than or equal to 1 antimicrobial sol in polyethylene endotracheal tube surface adhesion (0), surface hardness (5H), stable sol precipitation, antifungal sol coating and polyethylene tightly attached to the endotracheal tube surface, both to ensure the catheter flexibility, and ensure that the coating is not easy to break in the fold, and the sol coating uniformity stable coating; infrared spectrum analysis showed that TEOS/KH570/MTES/Ag-TiO2 (15/6/1/1.0) antibacterial sol cross-linking and stability, to ensure the coating composition to form a whole, not easy to fall off; the coating surface morphology analysis showed that the coating surface is very smooth, small particles and phase separation, surface coating compact network structure, stable without separation, to ensure the smooth coating catheter; coating light transmission detection showed an average transmittance of 90%, and has the antireflection effect; ICP-MS test, silver ion release almost Negligible, that antibacterial coating tube has antibacterial properties and good stability. The antibacterial test showed that the TiO2 Ag antibacterial coating the endotracheal tube with a clear antibacterial effect, can kill Pseudomonas aeruginosa, Staphylococcus aureus, Escherichia coli, antibacterial agent in different concentration. Different intensity of sterilization antimicrobial coated endotracheal tube coating the TiO2 Ag content is higher, is stronger antibacterial properties, the concentration reached 1% after sterilization are in saturated state, can almost completely kill all bacteria, 1% Ag / TiO2 antibacterial coated endotracheal tube is the lowest concentration of effective antibiotic. Oral mucosa irritation test showed that 1% Ag / TiO2 antimicrobial coated endotracheal tube extract on hamster oral mucosa without stimulation reaction; pyrogen test showed that the highest temperature liquid extraction tube coating 3H were 0 DEG c, 0 C, 0.03 C, the total temperature is 0.03 DEG C; hemolysis test showed absorbance of coated pipe is 0.016,0.014,0.021, the average value of 0.0170, calculated the hemolysis rate was 0.7512%; acute toxicity test showed that coating tube extracts had no animal died in the whole test process, and no abnormal reaction, morphology and cytotoxicity in vitro the test of normal cell, adherent growth is good, in the cytoplasm of discrete particles, no cell lysis, as non-toxic, determination of grade 0, the relative cell proliferation rates were greater than 100%.
Conclusion the TEOS/KH570/MTES/Ag-Ti02=15/6/1 is less than or equal to 1 and 1% concentration ratio of Ag / TiO2 antibacterial coating characterization of endotracheal tube the preparation of excellent, excellent antibacterial properties, biocompatibility and biological safety can be satisfied, pre clinical trials for prevention of VAP catheter as candidate.

【学位授予单位】:浙江中医药大学
【学位级别】:博士
【学位授予年份】:2014
【分类号】:R563.1

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