茶黄素纳米粒雾化吸入对大鼠炎性气道黏蛋白5AC的调控

发布时间：2018-01-05 01:03

  本文关键词：茶黄素纳米粒雾化吸入对大鼠炎性气道黏蛋白5AC的调控　出处：《重庆医科大学学报》2016年07期 　论文类型：期刊论文

  更多相关文章： 茶黄素 黏蛋白AC 纳米粒 雾化吸入

【摘要】：目的:探讨高频雾化吸入茶黄素乳酸-羟基乙酸共聚物(poly lactic-co-glycolic acid,PLGA)纳米粒对炎性气道黏蛋白5AC(mucoprotein 5AC,MUC5AC)的作用。方法:通过改良法制备茶黄素PLGA纳米粒,用粒径分析仪检测纳米粒平均粒径,并进行包封率和载药量测定及体外释放试验。将SD大鼠分为3组:对照组(A组)、香烟吸入组(B组)及干预组(C组)。RT-PCR检测各组黏蛋白5AC m RNA含量;酶联免疫吸附测定法(ELISA)观察MUC5AC蛋白表达的变化,并进行组化对比大鼠气道组织黏蛋白变化情况。结果:制备的茶黄素PLGA纳米粒性质稳定,测得其的平均粒径为(108.24±2.86)nm,适宜雾化吸入。香烟吸入组MUC5AC的蛋白表达明显升高,较对照组有显著性差异(P=0.000),而给予茶黄素PLGA纳米粒处理后,与香烟组比,指标明显下调(P=0.001)。MUC5AC m RNA表达的RT-PCR电泳结果也有类似结果。气道组织化学PAS染色也提示雾化吸入茶黄素PLGA纳米粒后,气道表面黏蛋白表达显著下降。结论:通过改良法制备茶黄素PLGA纳米粒性质稳定,大小均匀;经雾化途径吸入可发挥抑制炎性气道的黏蛋白5AC的高分泌作用,为茶黄素的开发应用提供了新的途径。
[Abstract]:Objective: to study the high frequency atomization inhalation of theaflavin-lactic acid-glycolic acid copolymer poly lactic-co-glycolic acid. The effect of PLGA nanoparticles on inflammatory airway mucoprotein 5ACmucoprotein 5ACMUC5AC. Methods: theaflavin PLGA nanoparticles were prepared by modified method. The average particle size was measured by particle size analyzer, encapsulation efficiency, drug loading and in vitro release test were performed. SD rats were divided into three groups: control group A). The content of 5AC m RNA was detected by RT-PCR in group C and group C in cigarette inhalation group (group B) and intervention group (group C). Enzyme linked immunosorbent assay (Elisa) was used to observe the expression of MUC5AC protein. Results: the properties of theaflavine PLGA nanoparticles were stable and the average diameter was 108.24 卤2.86 nm. The expression of MUC5AC protein in the cigarette inhalation group was significantly higher than that in the control group (P < 0.05), and the protein expression in the cigarette inhalation group was significantly higher than that in the control group (P < 0.05). However, after the treatment of theaflavin PLGA nanoparticles. Compared to cigarettes. Index significantly downgraded P0. 001). The results of RT-PCR electrophoresis of MUC5AC m RNA were similar. The airway histochemical PAS staining also suggested that the aerosol inhalation of theaflavin PLGA nanoparticles. Conclusion: the properties of theaflavine PLGA nanoparticles prepared by modified method are stable and uniform. Inhaled by atomization pathway can inhibit the hypersecretion of mucin 5AC in inflammatory airway, which provides a new way for the development and application of theaflavine.
【作者单位】： 重庆市中医院呼吸科;
【基金】：重庆市科委基金资助项目(编号:cstc2013jcyjA1062)
【分类号】：R56
【正文快照】： 慢性气道炎症是导致支气管哮喘、慢性阻塞性肺疾病(chronic obstructive pulmonary diseases,COPD)等临床常见呼吸系统疾病的主要病理基础。长期的炎症反应损伤气道上皮,进而使气道上皮杯状细胞、黏膜下腺体增生,纤毛损伤,黏液分泌增多,黏液屏障破坏,引起气流阻塞,逐渐形成COP，

本文编号：1380867