结核分枝杆菌抗原特异性白细胞介素-22在结核性胸膜炎鉴别诊断中的作用研究

发布时间：2018-01-15 11:04

本文关键词：结核分枝杆菌抗原特异性白细胞介素-22在结核性胸膜炎鉴别诊断中的作用研究　出处：《中国人民解放军医学院》2014年硕士论文　论文类型：学位论文

【摘要】：目的:通过分析结核性与恶性胸液单个核细胞（Pleural fluid mononuclear cells,PFMCs）在受到结核分枝杆菌特异性抗原肽ESAT-6（early secreted antigenic target-6）和CFP-10（culture filtrate protein-10）刺激后表达白细胞介素-22（Interleukin-22，IL-22）的特点，探索结核性和恶性胸水的鉴别诊断新方法。方法：采用e-Bioscience的IL-22和干扰素-γ（Interferon-γ，IFN-γ）试剂盒和流式细胞术微球阵列法，对52例结核性胸水和35例恶性胸水中经结核分枝杆菌特异性抗原肽刺激后PFMCs所表达的IL-22和IFN-γ浓度进行测定，并作统计学分析。结果:经结核杆菌特异抗原肽刺激，，结核性胸水上清中PFMC的IL-22表达水平显著升高（P＜0.05），且高于恶性胸水（P＜0.05）。结核性胸膜炎的结核杆菌特异性IL-22在胸液上清PFMC中的表达水平与IFN-γ显著相关(相关系数r=0.3485，P=0.0320)。ROC曲线显示，结核杆菌特异性IL-22用于结核性胸膜炎的诊断敏感性、特异性和准确性分别为90.38%、80.56%和85.23%，其诊断效能与IFN-γ相当(P＞0.05)。结论：结核分枝杆菌抗原特异性IL-22可作为鉴别诊断胸腔积液的新指标。
[Abstract]:Objective : To investigate the characteristics of interleukin - 22 ( IL - 22 ) after stimulation of Mycobacterium tuberculosis specific antigen peptide ( ESAT - 6 ) and P - 10 ( culture filtrate protein - 10 ) by analyzing tuberculous and malignant pleural fluid mononuclear cells ( PFs ) , and to explore new methods for differential diagnosis of tuberculous and malignant pleural effusion . Methods : The concentrations of IL - 22 and IFN - 纬 in 52 cases of tuberculous pleural effusion and 35 cases of malignant pleural effusion were measured with e - bioscience IL - 22 and interferon - 纬 ( interferon - 纬 , IFN - 纬 ) kit and flow cytometry . Results : The level of IL - 22 expression of PFMC was significantly increased ( P & lt ; 0.05 ) in the tuberculous pleural fluid by Mycobacterium tuberculosis specific antigen peptide stimulation ( P & lt ; 0.05 ) , and the expression level of TB - specific IL - 22 in pleural fluid supernatant was significantly correlated with IFN - 纬 ( r = 0.3485 , P = 0.0320 ) . ROC curves showed that the diagnostic sensitivity , specificity and accuracy of mycobacterium tuberculosis specific IL - 22 were 90.38 % , 80.56 % and 85.23 % , respectively , and their diagnostic efficacy was comparable with that of IFN - 纬 ( P > 0.05 ) . Conclusion : Mycobacterium tuberculosis antigen - specific IL - 22 can be used as a new index for differential diagnosis of pleural effusion .

【学位授予单位】：中国人民解放军医学院
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R521.7

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