氢硫酸钠抑制脂多糖引起的A549细胞损伤

发布时间：2018-01-17 13:34

  本文关键词：氢硫酸钠抑制脂多糖引起的A549细胞损伤　出处：《细胞与分子免疫学杂志》2016年04期 　论文类型：期刊论文

  更多相关文章： 急性肺损伤 脂多糖 氢硫酸钠(Na HS) 肺上皮细胞 闭锁小带蛋白

【摘要】：目的观察氢硫酸钠(Na HS)对脂多糖(LPS)诱导的A549肺癌细胞损伤的影响并探讨其可能机制。方法 A549细胞分为对照组、LPS处理组、Na HS处理组和LPS联合Na HS处理组。处理24 h后,采用ELISA测定培养液中C反应蛋白(CRP)的含量;MTT法与乳酸脱氢酶(LDH)法测定细胞损伤情况;测定A549细胞单层模型的跨上皮电阻(TER)值;免疫荧光染色法测定A549细胞中闭锁小带蛋白1(ZO-1)的分布情况。结果对照组与Na HS处理组比较,CRP的水平、TER值、细胞活力和ZO-1表达与分布情况无显著性差异;与对照组相比,LPS处理组中的CRP水平明显升高、TER值显著减少、细胞活力下降、细胞膜ZO-1表达减少且边界不清;与LPS处理组相比,LPS联合Na HS处理组中的CRP水平明显降低、TER值增加、细胞活力增加,ZO-1在细胞膜处的表达增强且分布情况部分恢复。结论 Na HS通过增强A549细胞活力、降低CRP水平、增加TER值并增加ZO-1表达抑制LPS诱导的肺损伤。
[Abstract]:Objective To observe the effect of sodium hydrogen sulfate (Na HS) on lipopolysaccharide (LPS) induced A549 damage of lung cancer cells and to explore its possible mechanism. Methods A549 cells were divided into control group, LPS treatment group, Na HS treatment group and LPS combined with Na HS group. After 24 h, determination of C reactive protein in culture using ELISA (CRP) content; MTT and lactate dehydrogenase (LDH) method for the determination of cell injury; transepithelial electrical resistance determination of A549 cell monolayer model (TER); Determination of A549 cell zonula occludens protein 1 immunofluorescence staining method (ZO-1). The distribution of the results of control group group compared with the Na HS treatment, CRP level, TER value, cell viability and ZO-1 expression had no significant difference with the distribution; compared with the control group, LPS treatment group, CRP levels were significantly increased, TER value decreased significantly, decreased cell viability, cell membrane expression of ZO-1 decreased and the boundary is not clear; compared with LPS group, LPS Lian Na HS in the CRP group were significantly decreased, TER value increased, the cell viability increased, the expression of ZO-1 in cell membrane enhanced recovery and distribution part. Conclusion Na HS enhances A549 cell viability, decrease the level of CRP, increase TER value and increase of ZO-1 lung injury induced suppression of LPS expression.

【作者单位】： 河北省人民医院感染性疾病科;河北省人民医院感染性疾病科胸外科公费医保办公室;天津医科大学总医院麻醉科;
【基金】：河北省医学科学研究重点课题计划(ZL20140267)
【分类号】：R563
【正文快照】： 急性肺损伤(acute lung injury,ALI)是指在感染、休克等致伤因素下,肺毛细血管内皮细胞和肺泡上皮细胞损伤造成弥漫性肺间质及肺泡水肿,这些变化导致肺容积减少、肺顺应性降低、严重的通气/血流比例失调,最终可引起急性低氧性呼吸功能不全[1-3]。目前,ALI的病死率大约为35%~40，

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