慢性阻塞性肺疾病患者和吸烟肺功能正常者体内HIF-1α表达及意义

发布时间：2018-02-03 06:43

本文关键词： 慢性阻塞性肺疾病低氧诱导因子-α 肺功能　出处：《细胞与分子免疫学杂志》2014年08期 　论文类型：期刊论文

【摘要】：目的探讨低氧诱导因子-1α(HIF-1α)在慢性阻塞性肺疾病(COPD)患者和吸烟肺功能正常者肺组织和血清中表达及其临床意义。方法肺组织标本取自手术治疗的周围型肺癌患者,共32例,检测术前肺功能,将其分为吸烟COPD稳定期组(COPD组,n=10),吸烟肺功能正常组(n=12),不吸烟肺功能正常组(n=10)。术前清晨空腹取血清,术后取癌旁肺组织,ELISA检测血清和肺组织匀浆HIF-1α含量,免疫组织化学方法检测肺组织HIF-1α的表达,并分析HIF-1α表达水平与肺功能指标的相关关系。结果 COPD稳定期组、吸烟肺功能正常组、不吸烟肺功能正常组患者血清HIF-1α含量分别为(73.25±6.12)pg/mL、(60.30±8.00)pg/mL、(47.03±8.43)pg/mL;吸烟肺功能正常组和COPD组明显高于不吸烟肺功能正常组(P0.01),COPD组明显高于吸烟肺功能正常组(P0.01)。COPD稳定期组、吸烟肺功能正常组、不吸烟肺功能正常组患者肺组织匀浆HIF-1α含量分别为(2.04±0.24)pg/μg、(1.67±0.34)pg/μg、(1.12±0.33)pg/μg;吸烟肺功能正常组和COPD组明显高于不吸烟肺功能正常组(P0.01),COPD组明显高于吸烟肺功能正常组(P0.01)。HIF-1α广泛表达于COPD组肺泡和气道上皮细胞、肺小动脉壁的炎症细胞以及巨噬细胞中。血清和肺组织匀浆中HIF-1α含量均与第1秒用力呼气容积与用力肺活量比(FEV1/FVC)、第1秒钟用力呼气容积占预计值百分比(FEV1%pred)呈显著负相关。结论 COPD患者和吸烟肺功能正常者肺组织和血清HIF-1α的表达增加,与气流受限有关。
[Abstract]:Objective to investigate the effect of hypoxia inducible factor-1 伪 (HIF-1 伪) on chronic obstructive pulmonary disease (COPD). Methods the expression and clinical significance of lung tissue and serum in patients with normal pulmonary function and smoking were collected from patients with peripheral lung cancer treated by surgery. The pulmonary function was measured in 32 patients before operation. The patients were divided into two groups: the smoking COPD stable phase group and the smoking normal lung function group were divided into two groups: the control group (n = 10) and the control group (n = 12). Serum levels of HIF-1 伪 in serum and lung homogenate were determined by Elisa after operation. Immunohistochemical method was used to detect the expression of HIF-1 伪 in lung tissue, and the correlation between the expression of HIF-1 伪 and pulmonary function index was analyzed. Results the expression of HIF-1 伪 was detected in stable COPD group and normal lung function group. The levels of serum HIF-1 伪 in non-smoking patients with normal pulmonary function were 73.25 卤6.12g / mL and 60.30 卤8.00g / mL, respectively. 47.03 卤8.43 PG / mL; The pulmonary function of smoking group and COPD group were significantly higher than that of non-smoking lung function group (P 0.01) and smoking lung function normal group (P 0.01). The content of HIF-1 伪 in lung homogenate of patients with normal lung function and normal lung function was 2.04 卤0.24 ng / 渭 g / 渭 g and 1.67 卤0.34 ng / 渭 g respectively. 1.12 卤0.33 g / 渭 g; The lung function of smoking group and COPD group were significantly higher than that of non-smoking group (P 0.01). The expression of P0.01U. HIF-1 伪 in COPD group was significantly higher than that in normal lung function group. HIF-1 伪 was widely expressed in alveolar and airway epithelial cells in COPD group. The contents of HIF-1 伪 in serum and lung homogenate in inflammatory cells and macrophages of pulmonary arterioles were higher than those in FEV 1 / FVC ratio of forced expiratory volume to forced vital capacity at 1 seconds. Forced expiratory volume per second as a percentage of expected value FEV1 pred). Conclusion the expression of HIF-1 伪 in lung tissue and serum of patients with COPD and normal pulmonary function was increased. Relating to airflow limitation.
【作者单位】：徐州大屯煤电公司中心医院;徐州医学院附属医院呼吸科;
【分类号】：R563.9
【正文快照】： 慢性阻塞性肺病(chronic obstructive pulmonarydisease,COPD)是一种以持续气流受限为特征的慢性气道疾病,病情呈进行性发展[1]。多数COPD患者均伴有不同程度的缺氧,长期慢性缺氧促进了肺组织结构破坏及肺血管的重塑。低氧诱导因子(hypoxia-inducible factor-1,HIF-1)是一种调

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