酶联免疫斑点试验联合结核抗原检测对艾滋病合并结核感染的临床诊断价值

发布时间：2018-02-14 18:57

本文关键词： 结核分枝杆菌 HIV/AIDS 结核抗原 ELISPOT　出处：《浙江中医药大学》2012年硕士论文　论文类型：学位论文

【摘要】：目的：探讨酶联免疫斑点试验联合结核抗原检测方法对AIDS合并结核患者的临床价值。方法：32例AIDS患者分成结核组和非结核组,对两组患者进行ELISPOT.结核抗原、血培养、痰涂片、PPD、结核抗体检测,分析ELISPOT联合结核抗原检测方法是否较其他结核检测方法更有优势。结果：结核组各种检测方法的阳性率分别为：血培养：7.1%、痰涂片：7.1%、PPD：14.3%、结核抗体：21.4%、结核抗原：28.6%、ELISPOT:85.7%、ELISPOT联合结核抗原检测(简称联合检测)：92.9%,联合检测与血培养、痰涂片、PPD、结核抗体、结核抗原检测方法比较均有统计学意义(P0.005),与ELISPOT检测法比较无统计学意义(P0.9)。非结核组各种检测方法的阳性率分别为：血培养：0、痰涂片：0、PPD：0、结核抗体：16.7%、结核抗原：11.1%、ELISPOT:0.联合检测法：11.1%。结核组与非结核组各种检测方法比较,仅有ELISPOT及联合检测方法有统计学意义(P0.0001),其他检测方法无统计学意义。结论：第一：AIDS合并结核感染患者,ELISPOT联合结核抗原检测或单独ELISPOT检测具有更高的阳性率和敏感性。AIDS患者怀疑结核感染无法确诊时,可应用ELISPOT联合结核抗原检测方法,避免临床漏诊,联合方法值得在临床推广第二：传统方法检测(血培养、痰涂片、PPD、结核抗体、结核抗原)在结核及非结核组无统计学差异,传统方法单独检测在HIV人群中诊断结核效果不显著。
[Abstract]:Objective: to investigate the clinical value of Elisa combined with tuberculosis antigen detection in AIDS patients with tuberculosis. Methods 32 patients with AIDS were divided into tuberculosis group and non-tuberculosis group. The two groups were treated with ElisPOT.TB antigen, blood culture, sputum smear, tuberculosis antibody detection, and whether ELISPOT combined with TB antigen detection method was superior to other TB detection methods. Results: the positive rates of blood culture, sputum smear and sputum smear were as follows: blood culture, sputum smear, sputum smear, sputum smear, sputum smear, sputum smear, sputum smear, sputum smear, sputum smear, tuberculosis antibody, tuberculosis antigen: 1. 28. 628. 8. 7. 7. 7. 8. 7. 7. 7. 7. The positive rates were: blood culture, sputum smear, tuberculosis antibody, sputum smear, sputum smear, tuberculosis antibody. The positive rates of various methods in non-tuberculosis group were: blood culture 0, sputum smear 0: 0, tuberculosis antibody: 16.7g, tuberculosis antigen 11.1%, ELISPOT: 0. The positive rates of each method were as follows: blood culture: 0, sputum smear: 0, tuberculosis antibody: 16.7g, tuberculosis antigen 11.1a, ELISPOT: 0.The positive rates of the methods were as follows: blood culture: 0, sputum smear: 0, TB antibody: 16.7. Test method: 11.1. comparison of various methods of detection between tuberculosis group and non-tuberculosis group, Only ELISPOT and combined detection methods had statistical significance (P 0.0001), while other methods had no statistical significance. Conclusion:. No. 1: AIDS patients with tuberculosis infection may use ELISPOT combined with tuberculosis antigen detection method to avoid clinical misdiagnosis if the positive rate and sensitivity of either Elispot combined with tuberculosis antigen detection or ELISPOT alone test are higher and higher. When AIDS patients suspected that tuberculosis infection could not be diagnosed, the method of ELISPOT combined with tuberculosis antigen detection can be used to avoid clinical misdiagnosis. Combined method is worth popularizing in clinic. Second, the traditional methods (blood culture, sputum smear, tuberculosis antibody, tuberculosis antigen) had no statistical difference between tuberculosis and non-tuberculosis group, but the traditional method alone in the diagnosis of tuberculosis in HIV population did not have significant effect.
【学位授予单位】：浙江中医药大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R446.6;R512.91;R521

【参考文献】