雌二醇缩短哮喘小鼠引喘潜伏期并促进气道炎症

发布时间：2018-02-15 05:18

  本文关键词： 雌二醇 哮喘 引喘潜伏期 气道炎症　出处：《细胞与分子免疫学杂志》2017年04期 　论文类型：期刊论文

【摘要】：目的观察雌二醇对哮喘小鼠引喘潜伏期及气道炎症的影响。方法雄性BALB/c小鼠随机分为正常对照组、卵清蛋白(OVA)诱发哮喘组、400μg/kg雌二醇处理组、400μg/kg雌二醇联合7 mg/kg他莫昔芬组,每组10只。OVA诱发哮喘组、雌二醇处理组、雌二醇联合他莫昔芬组通过OVA致敏、激发建立哮喘模型,正常对照组以生理盐水替代。雌二醇处理组、雌二醇联合他莫昔芬组于每次激发前4 h给予400μg/kg雌二醇腹腔注射,雌二醇联合他莫昔芬组在注射雌二醇前30 min给予7 mg/kg他莫昔芬腹腔注射。正常对照组、OVA诱发哮喘组腹腔注射生理盐水作对照。于末次激发后24 h,测定各组小鼠引喘潜伏期。收集支气管肺泡灌洗液(BALF)检测白细胞总数并进行嗜酸性粒细胞和淋巴细胞分类计数,ELISA检测BALF中白细胞介素4(IL-4)、IL-13水平。HE染色观察肺组织病理变化。结果与正常对照组比较,OVA诱发哮喘组、雌二醇处理组、雌二醇联合他莫昔芬组引喘潜伏期均缩短;雌二醇处理组较OVA诱发哮喘组、雌二醇联合他莫昔芬组引喘潜伏期缩短;OVA诱发哮喘组与雌二醇联合他莫昔芬组引喘潜伏期无显著性差异。与正常对照组比较,OVA诱发哮喘组、雌二醇处理组、雌二醇联合他莫昔芬组IL-4、IL-13水平升高;雌二醇处理组较OVA诱发哮喘组、雌二醇联合他莫昔芬组IL-4、IL-13水平升高;OVA诱发哮喘组与雌二醇联合他莫昔芬组IL-4、IL-13水平相当。与正常对照组比较,OVA诱发哮喘组、雌二醇处理组、雌二醇联合他莫昔芬组BALF白细胞总数及嗜酸性粒细胞、淋巴细胞分类计数增加;雌二醇处理组较OVA诱发哮喘组、雌二醇联合他莫昔芬组白细胞总数及嗜酸性粒细胞、淋巴细胞分类计数增加;OVA诱发哮喘组与雌二醇联合他莫昔芬组比较,白细胞总数及嗜酸性粒细胞、淋巴细胞分类计数无显著性差异。正常对照组小鼠气道黏膜无充血肿胀,气道周围无炎性细胞浸润;OVA诱发哮喘组、雌二醇联合他莫昔芬组小鼠气道黏膜充血水肿,支气管壁及周围有较多炎细胞浸润;雌二醇处理组较OVA诱发哮喘组、雌二醇联合他莫昔芬组上述改变更为严重。结论雌二醇能缩短哮喘小鼠引喘潜伏期,促进气道炎症细胞浸润及IL-4、IL-13的表达。
[Abstract]:Objective to observe the effects of estradiol on asthmatic latency and airway inflammation in asthmatic mice. Methods male BALB/c mice were randomly divided into control group and ovalbumin ovalbumin induced asthma group (400 渭 g / kg estradiol plus 7 mg/kg tamoxifen). 10 rats in each group were sensitized with estradiol, estradiol and tamoxifen by OVA, and the normal control group was replaced by normal saline. The estradiol combined with tamoxifen group was injected intraperitoneally with 400 渭 g / kg estradiol 4 hours before each stimulation. The estradiol combined with tamoxifen group was intraperitoneally injected with tamoxifen for 7 mg/kg at 30 min before estradiol injection. The asthmatic group induced by normal control group was treated by intraperitoneal injection of normal saline 30 min before injection of estradiol. 24 hours after the last challenge, the dose of tamoxifen in each group was measured. Bronchoalveolar lavage fluid (BALF) was collected to detect the total number of leukocytes and eosinophilic granulocytes and lymphocytes. Elisa was used to detect IL-13 level in BALF. The pathological changes of lung tissue were observed by HE staining. Normal control group compared with OVA induced asthma group, In estradiol group, estradiol combined with tamoxifen group, the incubation period of asthma induced by estradiol and tamoxifen was shortened, and the asthma induced by estradiol group was higher than that in OVA group. There was no significant difference between estradiol combined with tamoxifen group and estradiol combined with tamoxifen group. The level of IL-4 and IL-13 in estradiol combined with tamoxifen group was higher than that in OVA induced asthma group. The levels of IL-4 and IL-13 in the estradiol combined with tamoxifen group were similar to those in the OVA induced asthma group and the estradiol combined tamoxifen group, and were compared with those in the OVA induced asthma group and the estradiol treated group. The total number of BALF leukocytes and eosinophils and the number of eosinophilic granulocytes in the estradiol combined with tamoxifen group were higher than those in the OVA induced asthma group and the estradiol combined with tamoxifen group. Compared with estradiol combined with tamoxifen group, there was no significant difference in total leukocyte count, eosinophilic granulocyte and lymphocyte classification count between OVA induced asthma group and OVA induced asthma group, but there was no hyperemia and swelling in airway mucosa of normal control group. There were no inflammatory cell infiltration around the airway. OVA-induced asthma group, estradiol combined with tamoxifen group mice airway mucosal congestion and edema, bronchial wall and surrounding inflammatory cell infiltration, estradiol treatment group than OVA induced asthma group, Conclusion estradiol can shorten the latent period of asthma induced asthma and promote the infiltration of airway inflammatory cells and the expression of IL-4 IL-13 in asthmatic mice.
【作者单位】： 徐州医科大学附属医院呼吸内科;
【分类号】：R562.25
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