地塞米松对哮喘小鼠气道平滑肌细胞中胸腺活化调节趋化因子表达的影响

发布时间：2018-03-03 19:18

本文选题：哮喘　切入点：胸腺活化调节趋化因子　出处：《山东医药》2012年05期 　论文类型：期刊论文

【摘要】：目的探讨胸腺活化调节趋化因子(TARC)在哮喘小鼠气道平滑肌细胞中的表达及地塞米松对TARC的影响。方法 36只小鼠随机分为对照组(A组)、哮喘模型组(B组)、地塞米松治疗组(C组),每组12只。以卵白蛋白OVA和氢氧化铝混悬液致敏及OVA激发建立哮喘小鼠模型。行支气管肺泡灌洗液(BALF)沉渣白细胞及嗜酸性粒细胞(EOS)计数,HE染色观察肺组织病理改变,应用酶联免疫吸附实验测定血清及BALF中TARC、IL-4的浓度,并用免疫组织化学的方法测定气道平滑肌细胞中TARC蛋白的表达量。结果 B组白细胞及EOS计数明显高于A、C组(P0.01),C组EOS计数高于A组(P0.05)。B组血清及BALF中TARC及IL-4的浓度显著高于A、C组(P0.01);C组血清、BALF中TARC的浓度低于B组(P0.01)。B组气道平滑肌细胞中TARC蛋白表达量较A、C组显著增高(P0.01)。小鼠血清、BALF中TARC浓度与IL-4浓度呈正相关(r=0.669、0.845,P均0.01)。结论 TARC在哮喘组小鼠肺组织气道平滑肌细胞中的蛋白表达量较正常组明显增多,地塞米松可能通过减少IL-4的分泌从而抑制TARC的表达。
[Abstract]:Objective to investigate the expression of thymus activation regulatory chemokine tact (TARC) in airway smooth muscle cells of asthmatic mice and the effect of dexamethasone on TARC. Methods Thirty-six mice were randomly divided into control group A, asthma model group B and dexamethasone. The asthmatic mouse model was established by sensitizing ovalbumin OVA and aluminum hydroxide suspension and stimulated by OVA. The leucocytes and eosinophils in the leucocytes and eosinophils of bronchoalveolar lavage fluid (BALF) were stained with HE. Observe the pathological changes of lung tissue, The concentration of IL-4 in serum and BALF was determined by enzyme-linked immunosorbent assay (Elisa). The expression of TARC protein in airway smooth muscle cells was determined by immunohistochemical method. Results the white blood cell and EOS count in group B was significantly higher than that in group A (P 0.01) and EOS in group C was higher than that in group A (P 0.05). Group B was significantly higher than group A in the concentration of TARC and IL-4 in serum and BALF. The expression of TARC protein in the airway smooth muscle cells in group B was significantly higher than that in group A (P 0.01). There was a positive correlation between TARC concentration and IL-4 concentration in serum of mice. Conclusion the expression of TARC in asthmatic asthmatic smooth muscle cells is positively correlated with the concentration of IL-4 (0.6690.845, P < 0.01). Conclusion the expression of TARC protein in the airway smooth muscle cells in group B is significantly higher than that in group A (P 0.01). Conclusion the expression of TARC in the airway smooth muscle cells of group B is significantly higher than that in group A (P 0.01). The expression of protein in the airway smooth muscle cells in the lung tissue of the mice in the control group was significantly higher than that in the normal group. Dexamethasone may inhibit the expression of TARC by reducing the secretion of IL-4.
【作者单位】：滨州医学院附属医院;
【基金】：山东省自然科学基金资助项目(Y2007C162)
【分类号】：R562.25

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