硫化氢对臭氧致小鼠气道炎症的影响

发布时间：2018-03-05 12:54

本文选题：硫化氢　切入点：臭氧　出处：《中国病理生理杂志》2016年10期 　论文类型：期刊论文

【摘要】：目的:研究硫化氢(H_2S)对臭氧(O_3)暴露所致小鼠气道炎症的影响,并探究其机制。方法:32只C57BL/6小鼠随机分为正常对照(control)组、O_3组、硫氢化钠(NaHS)+O_3组以及NaHS组。第1、3、5天将O_3组和NaHS+O_3组小鼠置于2.14 mg/m~3O_3环境中暴露3 h,同时control组和NaHS组置于新鲜空气中暴露3 h。每次暴露前30 min,NaHS+O_3组和Na HS组小鼠腹腔注射Na HS(14μmol/kg)。末次暴露24 h后测定小鼠气道反应性,收集支气管肺泡灌洗液用于炎性细胞计数和总蛋白测定,收集肺组织标本行HE染色观察形态改变。测定肺组织中IL-6、IL-8、丙二醛(MDA)和NF-κB p65蛋白水平。结果:与control组相比,O_3组的气道反应性、炎性细胞数、总蛋白浓度和炎症评分,以及IL-6、IL-8、MDA和NF-κB p65蛋白水平明显增高,NaHS+O_3组则明显低于O_3组。结论:H_2S显著减轻了O_3暴露所致气道炎症反应,可能与抑制脂质过氧化和降低NF-κB表达相关。
[Abstract]:Objective: to study the effect of HSCH _ 2S) on airway inflammation in mice exposed to ozone O _ (O _ (3)) and its mechanism. Methods: thirty two C57BL / 6 mice were randomly divided into control group (n = 3) and control group (n = 3). The mice of group O _ S _ 3 and NaHS _ O _ S _ 3 were exposed to 2.14 mg/m~3O_3 for 3 hours, while the control and NaHS groups were exposed to fresh air for 3 h. 30 minutes before each exposure, the mice of group 3 and group NaHS were exposed to intraperitoneal cavity. The airway reactivity of mice was measured 24 h after the last exposure to Na HS(14 渭 mol / kg. Bronchoalveolar lavage fluid was collected for inflammatory cell count and total protein determination. Lung tissue specimens were collected for HE staining to observe morphological changes. The levels of IL-6 IL-8, malondialdehyde (MDA) and NF- 魏 B p65 protein in lung tissue were determined. Results: compared with control group, the airway reactivity, inflammatory cell number, total protein concentration and inflammatory score were measured. The levels of MDA and NF- 魏 B p65 protein in IL-6 + IL-8 group were significantly higher than those in group O3. Conclusion the level of IL-8MDA and NF- 魏 B p65 protein in group 3 is significantly lower than that in group O3. Conclusion\\\%\%\\\;
【作者单位】：广州医科大学生理教研室;
【基金】：国家自然科学基金资助项目(No.81470205)
【分类号】：R56

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