瘦素促进低氧状态下大鼠气道平滑肌细胞增殖及HIF-1α、NF-κB表达

发布时间：2018-03-07 02:25

本文选题：瘦素　切入点：气道平滑肌细胞　出处：《细胞与分子免疫学杂志》2015年01期 　论文类型：期刊论文

【摘要】：目的研究瘦素对低氧状态下大鼠气道平滑肌细胞(ASMC)增殖及缺氧诱导因子1α(HIF-1α)、核因子-κB(NF-κB)表达的影响。方法分常氧、低氧2种状态体外培养大鼠ASMC,按随机数字表法将低氧组细胞分为低氧组、50μg/L瘦素联合低氧组(L50组)、100μg/L瘦素联合低氧组(L100组)、200μg/L瘦素联合低氧组(L200组)、200μg/L瘦素联合低氧和瘦素受体抗体组(ob-R抗体组)。各组均孵育24 h,CCK-8法检测细胞增殖率,反转录PCR检测HIF-1α、NF-κB的mRNA的表达,Western blot法检测HIF-1α、NF-κB的蛋白表达。结果与常氧组相比,各低氧组细胞增殖活性明显增强;与低氧组相比,L50、L100、L200组细胞增殖活性增强,并与浓度呈正相关(r=0.992);与L50、L100、L200组相比,ob-R抗体组细胞增殖明显降低。与常氧组相比,各低氧组HIF-1α、NF-κB的mRNA及蛋白表达增加;与低氧组相比,L50、L100、L200组HIF-1α、NF-κB的mRNA及蛋白表达增加,并与浓度呈正相关;与L50、L100、L200组相比,ob-R抗体组HIF-1α、NF-κB的mRNA及蛋白表达降低。结论瘦素能促进低氧状态下ASMC增殖,并且能够促进HIF-1α、NF-κB的表达。
[Abstract]:Objective to study the effects of leptin on the proliferation of rat airway smooth muscle cells (ASMCs) and the expression of hypoxia inducible factor-1 伪 -HIF-1 伪, nuclear factor- 魏 B, NF- 魏 B in cultured rat airway smooth muscle cells under hypoxia. ASMCs were cultured in vitro in two hypoxic states. The cells of hypoxia group were randomly divided into hypoxia group (n = 50 渭 g / L leptin + hypoxia group) and hypoxia group (n = 100 渭 g / L leptin + hypoxia group). The cell proliferation rate was detected by CCK-8 method after incubation for 24 h in the neob-R antibody group. Reverse transcription PCR was used to detect the expression of HIF-1 伪 -NF- 魏 B mRNA. Western blot assay was used to detect the protein expression of HIF-1 伪 -nf- 魏 B. results compared with the normoxic group, the proliferative activity of the cells in each hypoxia group was significantly increased, while that in the hypoxia group was significantly higher than that in the hypoxia group. Compared with the normal group, the expression of mRNA and protein of HIF-1 伪 -NF- 魏 B was increased in all hypoxia groups, and the mRNA and protein expression of HIF-1 伪 NF- 魏 B in L50 L100 L200 group was higher than that in hypoxia group, and the expression of HIF-1 伪 NF- 魏 B protein was positively correlated with the concentration of HIF-1 伪 -kappa B in L50 L100 L200 group, and the expression of HIF-1 伪 NF- 魏 B protein in L50 L100 L200 group was significantly higher than that in normoxic group, and the expression of HIF-1 伪 NF- 魏 B protein in L50 L100 L200 group was significantly higher than that in normal hypoxia group. The expression of mRNA and protein of HIF-1 伪 -NF- 魏 B in HIF-1 伪 -NF- 魏 B antibody group was lower than that in L50 and L100 L200 group. Conclusion leptin can promote the proliferation of ASMC and the expression of HIF-1 伪 -NF- 魏 B in hypoxic condition.
【作者单位】：徐州医学院附属医院呼吸内科;大屯煤电集团责任(有限)公司中心医院内科;
【分类号】：R562.25

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