放射性肺损伤中Foxp3，RORγt和IL-17的网络研究及恩度的调控作用

发布时间：2018-03-11 19:20

本文选题：放射性肺炎　切入点：Foxp3　出处：《华中科技大学》2013年硕士论文　论文类型：学位论文

【摘要】：一、目的研究小鼠放射性肺损伤模型中Foxp3,RORγt和IL-17表达水平的动态变化，探讨放射性肺损伤可能的新机制，探讨恩度调节放射性肺损伤中的因子表达情况及可能机制，寻找防治放射性肺损伤的有效途径。二、方法 1.建立放射性肺损伤模型和探讨胸部照射对Foxp3,RORγt,IL-17及IL-17F表达的影响：小鼠60只随机分为2组:⑴空白对照组（NT组），⑵单纯照射组(RT组)。分别于照射后相应时间点从每组中随机选出3只小鼠取出肺组织。左肺行免疫组化，Masson染色和HE染色，右肺行western-blot和RT-PCR，检测Foxp3,RORγt,IL-17,IL-17F,TGF-β1和TNF-α因子表达情况。2.探讨恩度对Foxp3,RORγt,IL-17和IL-17F因子的调控作用和对放射性肺损伤的防治作用：小鼠120只随机分为4组:⑴空白对照组（NT组），⑵单纯照射组(RT组)，⑶照射+恩度组(RT+EN组)，⑷单纯恩度组(EN组)。相应时间取出肺组织。左肺行免疫组化，Masson染色和HE染色，右肺做western-blot和RT-PCR。三、结果 ⑴在多个时间点RT组小鼠肺组织HE染色见II～III级肺泡炎，NT组未见肺泡炎表现，Masson染色见胶原沉积明显较NT组增多。在多个时间点RT组小鼠肺组织Foxp3和RORγt的mRNA相对含量均较NT组小鼠升高，，具有统计学差别。照射后4周和8周RT组小鼠肺组织Foxp3，RORγt和IL-17F蛋白的含量较NT组高，有统计学差异。RT组TGF-β1、TNF-α的mRNA和蛋白的含量在多个时间点较NT组增多，具有统计学差异。 ⑵多个时间点RT+EN组小鼠肺组织HE染色和Masson染色较RT组轻，RT+EN组Foxp3、RORγt、TNF-α和TGF-β1的mRNA相对含量均较同一时间点RT组减少，具有统计学差别。4周和8周RT组小鼠肺组织TGF-β1和RORγt蛋白的含量较RT组减少，有统计学差异。8周RT组小鼠肺组织Foxp3和TNF-α蛋白的含量较RT组减少，有统计学差异。四、结论在放射性肺损伤模型中，TGF-β1、TNF-α、Foxp3和RORγt在mRNA和蛋白水平显著升高。Foxp3和RORγt成为放射性肺损伤发生新的预测因子，并提供新的可能机制。恩度能够下调由于放射性肺损伤引起的TGF-β1、TNF-α、Foxp3和RORγt。
[Abstract]:I. purpose. To study the dynamic changes of the expression levels of Foxp3P ROR 纬 t and IL-17 in the model of radiation induced lung injury in mice, to explore the possible new mechanism of radiation lung injury, and to explore the expression of factors and possible mechanisms in the regulation of radiation induced lung injury by Endor. To find an effective way to prevent and cure radiation lung injury. II. Methodology. 1. To establish a model of radiation induced lung injury and to investigate the effects of chest irradiation on the expression of IL-17 and IL-17F in Foxp3 ROR 纬 tng: 60 mice were randomly divided into 2 groups: control group, control group (n = 2), control group (n = 2) and control group (n = 2). Three mice were randomly selected to take out the lung tissue. The left lung was stained with Masson immunohistochemical staining and HE staining. The expression of TGF- 尾 1 and TNF- 伪 factor TGF- 尾 1 and TNF- 伪 were detected by western-blot and RT-PCR in right lung. The lung tissue was removed from the lung tissue in the same time. The left lung was stained with Masson immunohistochemical staining and HE staining. Western-blot and RT-PCR were performed in the right lung. III. Results. 1HE staining in lung tissue of RT mice at multiple time points showed that there was no alveolitis manifestation in II~III grade alveolitis NT group and collagen deposition in lung tissue of RT group was significantly higher than that in NT group, and the relative mRNA content of Foxp3 and ROR 纬 t in lung tissue of RT group was higher than that of NT group at different time points. All of them were higher than those in NT group. The contents of Foxp3ROR 纬 t and IL-17F protein in lung tissue of RT group were higher than that of NT group at 4 and 8 weeks after irradiation, and the mRNA and protein contents of TGF- 尾 1 and TNF- 伪 in RT group were higher than those in NT group at 4 and 8 weeks after irradiation, and the difference was statistically significant. 2 the relative contents of mRNA in lung tissue of RT en group were lower than that of RT en group, and the relative contents of TNF- 伪 and TGF- 尾 1 in lung tissue of RT en group were lower than those of RT group at the same time point, and the relative contents of TGF- 尾 1 and TNF- 伪 in lung tissue of RT en group were lower than those of RT en group at the same time point. The contents of TGF- 尾 1 and ROR 纬 t protein in lung tissue of RT group were significantly lower than those of RT group at week 4 and 8, and the contents of Foxp3 and TNF- 伪 protein in lung tissue of RT group were lower than those of RT group at week 8. There was statistical difference between RT group and RT group in the content of TGF- 尾 1 and TNF- 伪 protein. IV. Conclusions. In radiation-induced lung injury model, TGF- 尾 1 TNF- 伪 -Foxp3 and ROR 纬 t increased significantly at the level of mRNA and protein. Foxp3 and ROR 纬 t became new predictors of radiation-induced lung injury and provided a new possible mechanism. Endox can down-regulate TGF- 尾 1TNF- 伪 Foxp3 and ROR 纬 t induced by radiation-induced lung injury.
【学位授予单位】：华中科技大学
【学位级别】：硕士
【学位授予年份】：2013
【分类号】：R563.8

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