HCMV急性间质性肺炎小鼠模型的建立

发布时间：2018-03-11 21:52

本文选题：人巨细胞病毒　切入点：小鼠模型　出处：《安徽医科大学学报》2014年09期 　论文类型：期刊论文

【摘要】：目的建立人巨细胞病毒(HCMV)急性间质性肺炎的小鼠模型。方法用5.5×105PFU的HCMV AD169株尾静脉注射6~8周SPF级BALB/c小鼠作为实验组,同时设立正常细胞作为对照组,分别于接种后第5天和第30天处死小鼠,无菌取小鼠肺组织,通过病毒分离、PCR检测病毒特定保守基因、免疫组化、Western blot和HE染色的方法,观察检测小鼠肺组织中HCMV及其组织病理变化。结果在感染后第5天和第30天实验组小鼠的肺组织中分离出了HCMV病毒;PCR检测到HCMV特异性早晚期基因IE和UL55DNA;Western blot、免疫组化检测到HCMV特异性早晚期蛋白IE和gB;HE染色证实肺组织有明显急性间质性肺炎的病理改变;对照组对应结果全为阴性。结论通过尾静脉注射HCMV AD169株病毒,在感染后第5天成功构建了HCMV急性间质性肺炎小鼠模型,该模型的建立有利于该种疾病的发病机制研究及相关抗病毒疫苗和药物的研发。
[Abstract]:Objective to establish a mouse model of acute interstitial pneumonia of human cytomegalovirus (HCMV). Methods SPF grade BALB/c mice with 5.5 脳 105 PFU HCMV AD169 strain were injected by tail vein for 68 weeks as experimental group, and normal cells were set up as control group. The mice were killed on the 5th and 30th day after inoculation. The lung tissues of the mice were collected aseptically. The specific conserved genes of the virus were detected by virus isolation and PCR, and the immunohistochemical staining was performed by Western blot and HE staining. HCMV and its histopathological changes in lung tissue of mice were observed. Results HCMV virus was isolated from lung tissue of experimental group on the 5th and 30th day after infection. HCMV specific genes IE and UL55 DNA were detected in the early and late stage of the mice, and the immunized group was immunized. The pathological changes of lung tissue in acute interstitial pneumonia were confirmed by HCMV specific early and late stage protein IE and HE staining. Conclusion the mouse model of HCMV acute interstitial pneumonia was successfully established by injecting HCMV AD169 virus into tail vein on the 5th day after infection. The establishment of the model is conducive to the pathogenesis of the disease and the development of related antiviral vaccines and drugs.
【作者单位】：安徽医科大学微生物学教研室;哥伦比亚大学病理与细胞生物学教研室;
【基金】：国家自然科学基金(编号:30872253) 安徽省科技厅科技攻关项目(编号:8010302179) 安徽省教育厅自然科学重点课题(编号:KJ2009A041Z)
【分类号】：R563.1;R-332

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