脂肪乳对内毒素诱导急性肺损伤大鼠肺组织白介素6分泌及肺泡上皮细胞凋亡的影响

发布时间：2018-03-13 06:01

本文选题：脂肪乳剂　切入点：静脉注射用　出处：《中国全科医学》2014年06期 　论文类型：期刊论文

【摘要】：目的比较3种脂肪乳对脂多糖(LPS)诱导的急性肺损伤(ALI)大鼠肺组织白介素6(IL-6)分泌及肺泡上皮细胞凋亡的影响。方法选取清洁级幼年雄性Sprague-Dawley(SD)大鼠100只,按随机数字表法分为对照组、模型组、英脱利匹特组、克林诺组和尤文组,各20只。连续7 d分别向对照组和模型组大鼠注射0.9%氯化钠注射液(生理盐水)、英脱利匹特组注射脂肪乳注射液(C14~24)(英脱利匹特)、克林诺组注射长链脂肪乳注射液(OO)(克林诺)、尤文组注射ω3鱼油脂肪乳注射液(尤文);并在取标本前8 h于大鼠气管内滴入生理盐水(对照组)或LPS溶液(其余4组,建立ALI模型)。观察各组大鼠肺组织的病理改变,测定肺组织IL-6 mRNA表达、肺泡灌洗液(BALF)中IL-6水平、肺泡上皮细胞凋亡指数(AI)。结果 (1)除对照组外,其余4组大鼠气管内喷洒LPS溶液后肺组织均可见明显的炎性反应。(2)模型组和英脱利匹特组大鼠肺组织中IL-6 mRNA表达及BALF中IL-6水平均高于克林诺组和尤文组(P0.05);但克林诺组和尤文组肺组织IL-6 mRNA表达及BALF中IL-6水平间无差异(P0.05)。(3)克林诺组、尤文组大鼠肺泡上皮细胞AI均低于模型组和英脱利匹特组(P0.05);而克林诺组和尤文组AI间无差异(P0.05)。结论克林诺和尤文可通过降低IL-6分泌而减轻ALI大鼠炎性反应,并能减少肺泡上皮细胞凋亡,具有一定的抗炎作用。
[Abstract]:Objective to compare the effects of three kinds of fat milk on interleukin 6 (IL 6) secretion and alveolar epithelial cell apoptosis in lung tissue of rats with acute lung injury induced by lipopolysaccharide (LPS). Methods 100 young male Sprague-Dawley SD rats of clean grade were randomly divided into control group. The model group, the Intiripite group, the Klino group and the Juve group, 20 rats in each group were injected with 0.9% sodium chloride injection (normal saline solution) for 7 consecutive days in the control group and model group. OOO (Klinoxin, Ewing group) was injected with omega 3 fish oil fat emulsion injection (Ewing), and the rats were injected with normal saline (control group) or LPS solution (the other 4 groups) 8 hours before the specimen was taken. ALI model was established. The pathological changes of lung tissue were observed, the expression of IL-6 mRNA, the level of IL-6 in alveolar lavage fluid (BALF), and the apoptosis index of alveolar epithelial cells were measured in each group. The expression of IL-6 mRNA and the level of IL-6 in BALF of the other four groups were higher than those of the Klinor group and Ewing group, but the expression of IL-6 mRNA in the lung tissue was higher than that in the Klinor group and Ewing group, but the expression of IL-6 mRNA in the lung tissue was higher than that in the Ewing group, but the expression of IL-6 mRNA in the lung tissue of the other four groups was higher than that in the control group. There was no difference in the expression of IL-6 mRNA and the level of IL-6 in BALF between Noir group and Ewing group. The AI of alveolar epithelial cells in Ewing group was lower than that in model group and IMD group, but there was no difference in AI between Klinor group and Ewing group. Conclusion Klinor and Ewing can attenuate the inflammatory response of ALI rats by decreasing the secretion of IL-6. It can reduce the apoptosis of alveolar epithelial cells and has anti-inflammatory effect.
【作者单位】：新乡医学院第一附属医院新生儿科;温州医科大学附属第一医院;
【分类号】：R563.8

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