紫花前胡苷抑制哮喘小鼠气道炎性反应和NF-κB信号传导通路

发布时间：2018-03-17 17:26

本文选题：紫花前胡苷　切入点：紫花前胡　出处：《基础医学与临床》2014年05期 　论文类型：期刊论文

【摘要】：目的观察紫花前胡苷抗过敏性哮喘鼠气道炎性反应作用。方法 BALB/c小鼠被随机分为对照、模型、紫花前胡苷和地塞米松4组。除对照组外,所有小鼠腹腔注射和雾化吸入卵清蛋白以致敏和诱导气道炎性反应。于每次雾化前1h,给紫花前胡苷组灌胃10 mg/kg紫花前胡苷;给地塞米松组腹腔注射1 mg/kg地塞米松。动物肺功能仪分析气道高反应性;细胞计数器和Diff-Quick染色计数支气管灌洗液中白细胞总数及分类;酶联免疫吸附法检测血清或BALF中炎性介质的水平;苏木精-伊红染色法观察气道病理改变;电泳迁移率和免疫印迹法检测肺组织NF-κВ信号通路中蛋白的活力和表达。结果与对照组比较,模型组呈现明显的气道炎性反应,气道反应性显著增高(P0.05);血清或BALF中IgE、IL-4、IL-5和IL-13的水平显著增加(P0.01);细胞系P65、p-P65的水平显著增加(P0.05);细胞质P65和IκBα显著减少,NF-κB DNA-结合力显著增加(P0.05)。与模型组比较,紫花前胡苷能够显著抑制气道炎性反应和气道高反应(P0.05);降低血清或BALF中IgE、IL-4、IL-5和IL-13的水平(P0.01);抑制细胞系P65、p-P65水平(P0.05);增加细胞质P65、IκBα蛋白和减弱NF-κB DNA-结合力(P0.05)。结论紫花前胡苷具有抗过敏性哮喘鼠气道炎性反应。
[Abstract]:Objective to observe the anti-inflammatory effect of Bupleurin on airway inflammation in allergic asthmatic rats. Methods BALB/c mice were randomly divided into 4 groups: control group, model group, and dexamethasone group. All mice were injected intraperitoneally and inhaled ovalbumin to sensitize and induce airway inflammatory response. In the dexamethasone group, 1 mg/kg dexamethasone was injected intraperitoneally. The airway hyperresponsiveness was analyzed by animal pulmonary function analyzer, the total number and classification of leukocytes in bronchial lavage fluid were counted by cell counter and Diff-Quick staining. The levels of inflammatory mediators in serum or BALF were detected by enzyme-linked immunosorbent assay (Elisa) and the airway pathological changes were observed by hematoxylin-eosin staining. Electrophoretic mobility and Western blotting were used to detect the activity and expression of protein in NF- 魏 N signaling pathway in lung tissue. Results compared with the control group, the model group showed obvious airway inflammatory response. The level of IL-4 IL-5 and IL-13 in serum or BALF was significantly increased, the level of P65 p-P65 was significantly increased, the level of cytoplasmic p65 and I 魏 B 伪 was significantly decreased, and the DNA-binding ability of NF-kappa B was significantly increased in the model group, compared with the model group, the level of IL-5 and IL-13 in serum or BALF was significantly increased. Bupleurin could significantly inhibit airway inflammatory response and airway hyperresponsiveness (P0.05N), decrease the levels of IL-4 IL-5 and IL-13 in serum or BALF, inhibit the level of P65 p-p65, increase P65 I 魏 B 伪 protein and decrease NF- 魏 B DNA-binding force (P0.05). Glycosides have anti-allergic asthma rats airway inflammatory response.
【作者单位】：安徽科技学院食品药品学院;
【分类号】：R562.25

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