高迁移率族蛋白B1对16HBE细胞血管内皮生长因子表达和分泌的影响

发布时间：2018-03-17 19:45

本文选题：支气管哮喘　切入点：高迁移率族蛋白B　出处：《郑州大学学报(医学版)》2015年01期 　论文类型：期刊论文

【摘要】：目的:探讨高迁移率族蛋白B1(HMGB1)对人支气管上皮细胞(16HBE)血管内皮生长因子(VEGF)表达和分泌的影响及可能调控机制。方法:16HBE按以下方法分组。1HMGB1不同质量浓度组:0、100、500、2 000μg/L HMGB1刺激16HBE 24 h。2HMGB1不同作用时间组:对照组,2 000μg/L HMGB1分别刺激16HBE 6、12、24h组。MTT检测上述各组干预后16HBE的细胞活力,实时荧光定量PCR检测16HBE VEGF mRNA表达水平的变化,ELISA检测细胞培养上清中VEGF水平。应用P38 MAPK通路特异性抑制剂SB-202190观察其对HMGB1诱导VEGF表达和分泌的影响。结果:随HMGB1作用浓度的增加和作用时间的延长,VEGF的表达和分泌呈上升趋势(P均0.05),但细胞活力无明显改变(P均0.05)。HMGB1刺激增加了16HBE细胞VEGF的表达和分泌,SB-202190几乎完全抑制了HMGB1诱导的VEGF的表达和分泌(P均0.05)。结论:HMGB1可能通过P38 MAPK通路介导支气管上皮细胞VEGF的表达和分泌。
[Abstract]:Objective: To investigate the effect of high mobility group protein B1 (HMGB1) on human bronchial epithelial cells (16HBE) and vascular endothelial growth factor (VEGF) expression and secretion and the possible regulating mechanism. According to the following methods: 16HBE grouping method.1HMGB1 groups: 0100500,2 000 g/L 16HBE 24 h.2HMGB1 HMGB1 stimulation of different time groups. The control group, 2000 g/L HMGB1 16HBE 6,12,24h.MTT group were stimulated to detect the group intervention 16HBE cell viability, real-time detection of 16HBE VEGF mRNA fluorescent quantitative PCR expression, ELISA detection of cell culture supernatant in VEGF level. The application of P38 MAPK specific inhibitor SB-202190 was observed on HMGB1 induced VEGF expression and secretion effect. Results: with increasing HMGB1 concentration and treatment time, the expression and secretion of VEGF increased (P < 0.05), but no significant changes in cell viability (P 0.05). HMGB1 stimulation increased the expression and secretion of VEGF in 16HBE cells. SB-202190 almost completely inhibited the expression and secretion of VEGF induced by HMGB1 (P all 0.05). Conclusion: HMGB1 may mediate the expression and secretion of VEGF in bronchial epithelial cells through P38 MAPK pathway.

【作者单位】：广西医科大学第一附属医院呼吸疾病研究所;
【基金】：广西研究生教育创新计划YCBZ2013014
【分类号】：R562.25

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