二次纳流电喷雾电离耦合超高分辨质谱检测人体呼出气中相对高分子量化合物

发布时间：2018-03-20 13:11

本文选题：人体呼出气　切入点：相对高分子量　出处：《高等学校化学学报》2017年05期 　论文类型：期刊论文

【摘要】：二次电喷雾电离源耦合超高分辨质谱(SESI-UHRMS)有望检出人体呼出气中分子量大于300的相对高分子量化合物,这些化合物的发现将有助于更准确地理解呼出气中挥发性有机化合物(VOCs)的来源、产生机制以及SESI源电离机理,更好地实现SESI-UHRMS的转化应用.本研究自组装nanoSESI源(尚无商业产品)耦合四极杆-静电场轨道阱质谱(最高质量分辨率1.2×10~5),考察了该装置对健康人体呼出气中分子量为300~500化合物的检出情况.结果表明,所搭建的nanoSESI-UHRMS装置检测人体呼出气的重现性好、灵敏度高,可检出数十种分子量为300~500的化合物.根据这些化合物在单次呼气过程中信号强度随时间变化的趋势,推断其来源分别为内源性和外源性;各化合物的元素组成主要包括C,H,N和O,环-双键当量(RDB)的均值为(4.5±3.1),表明检出的化合物可能为醛酮或不饱和脂肪酸,容易在SESI源中被电离.本研究初步验证了自组建nanoSESI-UHRMS检测人体呼出气中相对高分子量化合物的可行性,为后续进一步开展方法应用奠定了基础.
[Abstract]:The secondary electrospray ionization source coupled with ultra-high resolution mass spectrometry (SESI-UHRMS) is expected to detect relative high molecular weight compounds with molecular weight greater than 300 in human exhalation. The discovery of these compounds will contribute to a more accurate understanding of the sources, production mechanisms and ionization mechanisms of volatile organic compounds (VOCs) in exhaled air, The conversion application of SESI-UHRMS is better realized. In this study, the self-assembled nanoSESI source (without commercial products) coupled with quadrupole electrostatic field orbital well mass spectrometry (maximum mass resolution 1.2 脳 10 ~ (5)) has been studied. The molecular weight of the device in the exhalation and exhalation of healthy human is investigated. The results show that, The nanoSESI-UHRMS device has good reproducibility and high sensitivity in detecting exhalation, and can detect dozens of compounds with molecular weight of 300 ~ 500. According to the trend of signal intensity changing with time during a single exhalation, It was inferred that the compounds originated from endogenous and exogenous sources respectively, and their elemental compositions mainly included Con HN and O, and the mean value of RDBs was 4.5 卤3.1, indicating that the compounds detected might be aldehydes, ketones or unsaturated fatty acids. It is easy to be ionized in SESI source. This study preliminarily verifies the feasibility of self-made nanoSESI-UHRMS for the detection of relative high molecular weight compounds in human exhaled air, which lays a foundation for the further application of this method.
【作者单位】：暨南大学质谱仪器与大气环境研究所广东省大气污染在线源解析系统工程技术研究中心;暨南大学第一附属医院;
【基金】：中央高校基本科研业务费专项资金(批准号:21615457)资助~~
【分类号】：O657.63;R56

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