NRAMP1基因多态性与青海汉族肺结核易感性的相关性研究

发布时间：2018-03-20 22:25

本文选题：NRAMP1基因　切入点：多态性　出处：《青海大学》2017年硕士论文　论文类型：学位论文

【摘要】：目的:研究NRAMP1基因多态性与青海地区汉族肺结核病易感性之间的关系,为青海地区汉族肺结核病分子生物学方面的研究提供一定的线索。方法:采用病例-对照研究。收集青海地区102例汉族肺结核患者及104例健康者的外周血。对rs17235409(D543N)、rs3731865(INT4)的2个位点采用实时荧光定量PCR—Taq Man探针法进行基因分型,对rs17235416(3'UTR)位点采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)的方法检测其基因型,分析上述3个位点的多态性与肺结核的关系。结果:rs17235409在病例组与对照组中G/G、G/A+A/A基因型频率分别为74.5%、25.5%和80.8%、19.2%,组间比较不具有统计学意义(X2=1.163,P=0.281);rs3731865在病例组与对照组中G/G、G/C+C/C基因型频率分别为72.5%、27.5%和72.1%、27.9%,组间比较不具有统计学意义(X2=0.005,P=0.945);rs17235416在病例组与对照组中TGTG/TGTG、TGTG/del+del/del基因型频率分别为74.5%、25.5%和87.5%、12.5%,组间比较具有统计学意义(X2=5.662,P=0.017),OR值(95%CI)为2.395(1.152-4.980);病例组中TGTG、del等位基因与对照组进行组间比较具有统计学意义(X2=9.878,P=0.002),OR值(95%CI)为2.771(1.439-5.338)。结论:NRAMP1基因的rs17235409、rs3731865位点多态性与青海汉族肺结核的易感性无关;rs17235416多态性是青海汉族肺结核的易感因素,TGTG(del)等位基因是青海汉族患肺结核的危险因素。
[Abstract]:Objective: to study the relationship between NRAMP1 gene polymorphism and susceptibility to pulmonary tuberculosis in Han nationality in Qinghai province. Methods: a case-control study was used to study the molecular biology of pulmonary tuberculosis in Qinghai Han nationality. The peripheral blood samples of 102 patients with pulmonary tuberculosis of Han nationality and 104 healthy persons in Qinghai were collected. Two loci were genotyped by real-time fluorescence quantitative PCR-Taq Man probe method. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used to detect the genotypes of rs17235416C3UTRs. Results the frequencies of G / G / A / A / A / A genotype in the case group and control group were 74.5% and 80.8%, respectively. There was no statistical significance between the two groups. The frequency of TGTG / TGTG / del del/del genotype was 74.525.5% and 87.525.5% and 87.512.5%, respectively. There was no significant difference between the two groups in the frequency of TGTG / TGTG / TGTG / del del/del, and the odds ratio (OR) of TGTG / TGTG / TGTG / del was 2.3951.152-4.980 in the case group, respectively. There was statistical significance in the comparison between the two groups. The OR value of TGTGndel allele was 2.3951.152-4.980 in the case group. Compared with the control group, the odds ratio (OR) was 2.771U 1.439-5.33380.Conclusion the rs17235409rs3731865 polymorphism of the 1: NRAMP1 gene is not related to the susceptibility of Qinghai Han people to pulmonary tuberculosis, rs17235416 polymorphism is the risk factor of Qinghai Han nationality pulmonary tuberculosis. It is the risk factor of pulmonary tuberculosis in Qinghai Han nationality.
【学位授予单位】：青海大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R521

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