RNA实时荧光恒温扩增检测技术在结核病诊断中的临床应用

发布时间：2018-03-21 10:24

本文选题：RNA实时荧光恒温扩增检测　切入点：结核分枝杆菌　出处：《中国卫生检验杂志》2014年22期 　论文类型：期刊论文

【摘要】：目的探讨RNA恒温扩增实时检测技术(simultaneous amplification and testing,SAT)在结核病诊断中的应用价值。方法采用RNA扩增实时检测试剂盒分别检测175例肺结核病患者、211例其他肺部疾病患者,并与痰菌培养及痰涂片比较检测的灵敏度和特异性,同时对培养阳性菌株进行鉴定。结果以临床诊断为"金标准",SAT检测结核病患者的敏感度为71.43%、特异度为99.05%,结核患者阳性检出率明显高于痰培养法和痰涂片法(P0.01);386例标本中有20例鸟-胞内分枝杆菌、4例脓肿分枝杆菌、1例戈登分枝杆菌、1例堪萨斯分枝杆菌、6例鸟分枝杆菌,SAT检测NTM结果均为阴性;SAT对涂阳培阳患者检出率为100%,涂阴培阳检出率78.4%,涂阴培阴检出率为56%,涂阳培阴检出率为35.7%。结论 RNA扩增实时检测技术检测肺结核具有快速方便、经济和较高的敏感度,值得用于临床结核筛查。
[Abstract]:Objective to explore the application value of RNA constant amplification and testing kit in the diagnosis of tuberculosis. Methods the RNA amplification real-time detection kit was used to detect 175 patients with pulmonary tuberculosis and 211 patients with other pulmonary diseases. The sensitivity and specificity of sputum culture and sputum smear were compared. Results the sensitivity and specificity were 71.43 and 99.05, respectively. The positive rate of tuberculosis was significantly higher than that of sputum culture and sputum smear in 386 cases. In this study, 20 cases of bird-endocytobacillus, 4 cases of mycobacterium abscess, 1 case of Mycobacterium Gordon, 1 case of Mycobacterium Kansan, 6 cases of Mycobacterium avium, 6 cases of Mycobacterium avium were detected. The NTM results were all negative. The positive rate of smear positive was 100%, and the rate of smear negative was 100%. The positive rate of Peiyang was 78.4%, the positive rate of smear yin was 56g and the positive rate of smear yang was 35.7.Conclusion RNA amplification real-time detection technique is rapid and convenient for detecting pulmonary tuberculosis. Economic and high sensitivity are worthy of clinical TB screening.
【作者单位】：浙江省中西医结合医院;浙江省疾病预防控制中心;
【基金】：杭州市卫生科技计划重点项目(2012Z009)
【分类号】：R521

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