Th17转录因子RORγt和BATF及Th17相关细胞因子在中性粒细胞亚型哮喘发病中的作用

发布时间：2018-03-30 03:27

本文选题：中性粒细胞亚型哮喘　切入点：辅助性T细胞　出处：《吉林大学学报(医学版)》2017年01期

【摘要】：目的:检测中性粒细胞亚型哮喘(NA)患者痰上清中辅助性T细胞17(Th17)特异性转录因子维甲酸相关核孤儿受体γt(RORγt)、B细胞转录激活因子(BATF)及外周血中Th17细胞相关细胞因子白细胞介素8(IL-8)、白细胞介素17(IL-17)和白细胞介素22(IL-22)表达水平,探讨其在NA发病中的作用。方法:以56例支气管哮喘患者为研究对象,4.5%生理盐水雾化诱导痰,经细胞MGG染色进行哮喘的炎症亚型分型,分为嗜酸性粒细胞亚型哮喘(EA)组(26例)和NA组(30例),以常规体检者为健康对照组(NC组,28人)。实时荧光定量PCR(RT-PCR)法检测痰上清中BATF和RORγt mRNA表达水平。空腹抽取各组研究对象肘静脉血10mL,Ficoll密度梯度离心后分离外周血单个核细胞(PBMC),ELISA法测定血清中IL-8、IL-17和IL-22蛋白表达水平,流式细胞术检测PBMC中Th17细胞(CD4+IL-17+细胞)的百分率。结果:与NC组和EA组比较,NA组患者血清中IL-8、IL-17和IL-22表达水平均明显升高(P0.05);与NC组比较,EA组患者血清中IL-8、IL-17和IL-22表达水平差异无统计学意义(P0.05)。与NC组和EA组比较,NA组患者痰上清中BATF和RORγt mRNA表达水平明显升高(P0.01)。与NC组和EA组比较,NA组患者PBMC中Th17细胞百分率均明显升高(P0.01);与NC组比较,EA组患者PBMC中Th17细胞百分率差异无统计学意义(P0.05)。结论:IL-17转录因子RORγt和BATF参与了NA患者气道炎症发生过程,且Th17细胞及其相关细胞因子IL-17和IL-22的异常表达与NA的全身反应有关联。
[Abstract]:Objective: to detect the specific transcription factor retinoic acid associated nuclear orphan receptor 纬 t(ROR 纬 twee B cell transcriptional activator (BATF) in the sputum supernatant of patients with neutrophil subtype Asthma (NAA) and the Th17 cell related cytokine white in peripheral blood. The expression levels of IL-8, IL-17 and IL-22 were observed. Methods: 56 patients with bronchial asthma were selected as subjects. Sputum was induced by 4.5% normal saline and subtypes of asthma were classified by cell MGG staining. There were 26 cases of eosinophil subtype asthma (EAA) group and 30 cases of na group. The expression of BATF and ROR 纬 t mRNA in sputum supernatant was detected by real-time fluorescence quantitative PCRRT-PCR method. The expression of IL-8 IL-17 and IL-22 protein in serum was determined by Elisa method in peripheral blood mononuclear cells isolated from 10 mL Ficoll density gradient centrifugation. Results: compared with NC group and EA group, the expression of IL-8, IL-17 and IL-22 in serum of PBMC patients were significantly higher than those of NC group and EA group, and the expression of IL-8 IL-17 and IL-22 in serum of EA group were significantly higher than that of NC group and EA group, respectively. The expression of BATF and ROR 纬 t mRNA in sputum supernatant in na group was significantly higher than that in NC group and EA group, but the percentage of Th17 cells in PBMC in na group was significantly higher than that in NC group and EA group, and in comparison with NC group and EA group, the percentage of Th17 cells in PBMC in na group was significantly higher than that in NC group. There was no significant difference in the percentage of Th17 cells in PBMC compared with that in EA group. Conclusion ROR 纬 t and BATF are involved in airway inflammation in na patients. The abnormal expression of IL-17 and IL-22 in Th17 cells and related cytokines was associated with the systemic reaction of na.
【作者单位】：吉林大学基础医学院病原生物学教研室;吉林省人民医院呼吸科;
【基金】：吉林省科技厅科技发展计划-科技创新人才培育计划资助课题(20150519015JH) 吉林大学大学生创新创业训练计划国家级项目资助课题(2015791156);吉林大学白求恩B计划资助课题(2012218)
【分类号】：R562.25

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