Toll样受体4及骨形成蛋白2表达在慢性阻塞性肺疾病患者血管重塑中的作用研究

发布时间：2018-04-02 08:38

本文选题：肺疾病　切入点：慢性阻塞性　出处：《中国全科医学》2014年36期

【摘要】：目的探讨肺血管中Toll样受体4(TLR-4)及骨形成蛋白2(BMP-2)的表达在慢性阻塞性肺疾病(COPD)患者血管重塑中的作用。方法肺组织标本取自青岛大学医学院附属医院胸外科2012年7月—2013年7月住院需要手术治疗的50例男性高分化肺鳞癌患者。根据是否患有COPD将患者分为COPD组和对照组,各25例。光学显微镜下观察肺动脉形态结构变化;采用图像分析系统测定管壁厚度占外径百分比(WT%)和血管壁横断面积占血管总面积百分比(WA%);免疫组化染色法检测肺动脉血管平滑肌细胞TLR-4、BMP-2、增殖细胞核抗原(PCNA)表达水平,并分析其与血管重塑的相关性。结果 COPD组患者炎症评分、WT%、WA%及肺动脉血管平滑肌细胞TLR-4、BMP-2、PCNA表达水平均高于对照组(P0.05)。COPD组患者肺动脉平滑肌细胞TLR-4、BMP-2水平与炎症评分、WT%、WA%及PCNA水平均呈正相关,PCNA水平与WT%、WA%、炎症评分均呈正相关(P0.05)。结论TLR-4、BMP-2的表达水平增加促进了炎性反应,诱导平滑肌细胞增殖,在COPD患者血管重塑过程中起到了重要作用。
[Abstract]:Objective to investigate the role of Toll like receptor 4 (TLR-4) and bone morphogenetic protein 2 (BMP-2) in vascular remodeling in patients with chronic obstructive pulmonary disease (COPD).Methods Pulmonary tissue samples were collected from 50 male patients with well-differentiated lung squamous cell carcinoma who were hospitalized in the Department of Thoracic surgery, affiliated Hospital of Qingdao University Medical College from July 2012 to July 2013.Patients were divided into COPD group and control group according to whether they had COPD.The morphological and structural changes of pulmonary artery were observed under optical microscope.Image analysis system was used to measure the percentage of wall thickness to external diameter (WTT) and transverse area of vascular wall to total vascular area. Immunohistochemical staining was used to detect the expression level of proliferating cell nuclear antigen (PCNA) in pulmonary artery vascular smooth muscle cells (TLR-4BMP 2).The relationship between it and vascular remodeling was analyzed.Results the expression of TLR-4BMP-2PCNA in pulmonary artery smooth muscle cells (TLR-4BMP-2PCNA) in COPD group was significantly higher than that in control group (P0.05). The level of TLR-4BMP-2 in pulmonary artery smooth muscle cells was positively correlated with the levels of WAW% and PCNA in COPD group.The symptom score was positively correlated with P0.05.Conclusion the increase of TLR-4 BMP-2 expression promotes inflammatory response, induces smooth muscle cell proliferation, and plays an important role in vascular remodeling in patients with COPD.
【作者单位】：山东省临沂市沂水县人民医院呼吸内科;青岛大学医学院附属医院呼吸内科;
【分类号】：R563.9

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