IL-13对气道上皮细胞凋亡的影响及其机制研究

发布时间：2018-04-02 11:23

本文选题：哮喘　切入点：IL-　出处：《免疫学杂志》2015年08期

【摘要】：目的观察IL-13对9HTE气道上皮细胞内Egr-1、Bax、Bcl-2 m RNA和蛋白表达的影响,探讨IL-13在气道上皮细胞凋亡中的作用及机制。方法将9HTE气道上皮细胞分为正常对照组、rh IL-13组及rh IL-13+m AIL-13组,用逆转录-多聚酶链反应(RT-PCR)法检测Egr-1、Bax、Bcl-2 m RNA表达水平,用免疫细胞化学(ICC)法检测Egr-1、Bax、Bcl-2蛋白表达水平。结果rh IL-13组Egr-1、Bax m RNA及蛋白表达显著高于正常对照组(P0.01),Bcl-2 m RNA及蛋白表达显著低于正常对照组(P0.01);rh IL-13+m AIL-13组Egr-1、Bax m RNA及蛋白表达显著低于rh IL-13组(P0.01),与正常对照组比较无显著性差异(P0.05),Bcl-2 m RNA及蛋白表达显著高于rh IL-13组(P0.01),与正常对照组比较无显著性差异(P0.05)。结论 IL-13可能通过Egr-1促进9HTE气道上皮细胞凋亡;m AIL-13可以中和IL-13所致的9HTE气道上皮细胞凋亡的作用。
[Abstract]:Objective to observe the effect of IL-13 on the expression of Bcl-2 m RNA and protein in 9HTE airway epithelial cells, and to explore the role and mechanism of IL-13 in the apoptosis of airway epithelial cells. Methods 9HTE airway epithelial cells were divided into two groups: normal control group, rh IL-13 group and rh IL-13 m AIL-13 group. The expression of Bcl-2 m RNA was detected by reverse transcription-polymerase chain reaction (RT-PCR). Results the expression of Egr-1Bax-Bax RNA and protein in rh IL-13 group was significantly higher than that in normal control group (P 0.01), Bcl-2 m RNA and protein expression were significantly lower than that in normal control group P0.01 IL-13 m AIL-13 and Egr-1 Bax m RNA and protein table. The expression of Bcl-2 m RNA and protein in rh IL-13 group was significantly higher than that in rh IL-13 group, and there was no significant difference between Rh IL-13 group and normal control group in the expression of Bcl-2 m RNA and protein. Conclusion IL-13 may promote 9HTE airway epithelium through Egr-1. Apoptotic AIL-13 can neutralize the apoptosis of 9HTE airway epithelial cells induced by IL-13.
【作者单位】：重庆市璧山区人民医院儿科;儿童发育疾病研究教育部重点实验室儿科学重庆市重点实验室重庆市儿童发育重大疾病诊治与预防国际科技合作基地;重庆医科大学附属儿童医院呼吸中心一病房;
【分类号】：R562.25

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