富硒酵母对大气细颗粒物致大鼠肺损伤的干预作用

发布时间：2018-04-10 20:25

本文选题：细颗粒物 + 肺损伤　；参考：《卫生研究》2017年02期

【摘要】：目的探讨富硒酵母对大气细颗粒物(PM_(2.5))致大鼠肺损伤的干预作用。方法 56只健康成年SD大鼠随机平均分为7组:生理盐水对照组(气管滴注生理盐水);PM_(2.5)染毒组(气管滴注PM_(2.5)混悬液,40 mg/kg,隔天一次,共3次);溶剂对照组(1%羧甲基纤维素灌胃);低、中、高剂量干预组(气管滴注PM_(2.5)混悬液,末次滴注24h后,分别灌胃给予富硒酵母8.75、17.5和35 mg/kg,每天1次,共7d);干预对照组(35 mg/kg富硒酵母灌胃)。末次灌胃24 h后,收集肺泡灌洗液(BALF),测定BALF中肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β)、丙二醛(MDA)、总蛋白(TP)含量及总超氧化物歧化酶(T-SOD)、谷胱甘肽过氧化物酶(GSH-Px)、乳酸脱氢酶(LDH)及碱性磷酸酶(AKP)活性,并计数细胞。未灌洗肺叶制作病理切片。结果与对照组相比,PM_(2.5)染毒组及低、中、高剂量干预组BALF中中性粒细胞百分比、TNF-α、IL-1β、TP及MDA含量,LDH及AKP活性增高,GSH-Px及T-SOD活性下降(P0.05)。与PM_(2.5)染毒组相比,干预组BALF中中性粒细胞百分比、TNF-α、IL-1β、TP及MDA含量,LDH及AKP活性降低,GSH-Px及T-SOD活性升高(P"0.05),随干预剂量增加,中性粒细胞百分比、TNF-α、IL-1β、TP及MDA含量,LDH及AKP活性降低,GSH-Px、T-SOD活性升高幅度增加。结论富硒酵母可适当减轻PM_(2.5)所致大鼠肺组织炎性损伤及氧化应激损伤。
[Abstract]:Objective to investigate the effect of selenium enriched yeast on lung injury induced by PMN 2.5 in rats.Methods 56 healthy adult Sprague-Dawley rats were randomly divided into 7 groups: saline control group (intratracheal drip of normal saline and PM2. 5) treated group (tracheal drip of PM2. 5) suspension 40 mg / kg, once every other day.The control group was fed with Se-enriched yeast for 35 mg/kg.24 hours after the last gavage,The contents of tumor necrosis factor- 伪 (TNF- 伪), interleukin-1 尾 (IL-1 尾), malondialdehyde (malondialdehyde) (MDAA), total protein (TTP) and the activities of total superoxide dismutase (T-SOD), glutathione peroxidase (GSH-PX), lactate dehydrogenase (LDH) and alkaline phosphatase (AKP) in BALF were measured and counted.The lung lobes were not lavaged to make pathological sections.The percentage of neutrophils in BALF in the intervention group decreased the activities of GSH-Px and T-SOD, and the contents of MDA, TP and MDA decreased, and increased with the increase of the dose of TNF- 伪, IL-1 尾, TNF- 伪, TNF- 伪, IL-1 尾, TNF- 伪, and the activity of T-SOD, and the activity of GSH-Px and T-SOD increased with the increase of the intervention dose.Percentage of neutrophilic granulocytes, TNF- 伪 -IL-1 尾, TP, and MDA content. LDH and AKP activities decreased and the activity of GSH-PxX T-SOD increased.Conclusion Selenium-enriched yeast can reduce the inflammatory injury of lung tissue and oxidative stress injury induced by PMSt 2.5 in rats.
【作者单位】：复旦大学公共卫生学院环境卫生学教研室公共卫生安全教育部重点实验室;
【基金】：国家自然科学基金(No.91543119)
【分类号】：R563

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