钾离子通道TASK-1 mRNA在大鼠呼吸中枢的分布

发布时间：2018-04-13 04:31

本文选题：TASK- + 钾离子通道　；参考：《神经解剖学杂志》2014年02期

【摘要】：目的:TASK-1是一种对生理范围内pH值敏感的二孔漏钾通道。本研究的目的是探讨TASK-1mRNA在脑干呼吸中枢的分布。方法:使用地高辛标记的寡核苷酸探针与脑干冰冻切片进行原位杂交,检测TASK-1mRNA在脑干呼吸相关神经元中的表达。通过免疫荧光组织化学方法来明确表达TASK-1mRNA的神经元类型。结果:TASK-1 mRNA在最主要的上气道运动神经核团-舌下神经核中有强表达。TASK-1 mRNA在延髓腹侧和背侧的中枢化学感受器神经核团上广泛分布且有较强表达,包括孤束核(nucleus tractus solitarius,NTS)、迷走神经背核(dorsal vagus motor nucleus,DMV)、前包氏复合体(pre-B錸tzinger complex,pre-B錸tC)、斜方体后核(retrotrapezoid nucleus,RTN)、尾段延髓中缝核团(caudal medullary raphe nuclei)以及蓝斑(the locus coeruleus,LOC)等部位。TASK-1 mRNA在脑桥呼吸组、桥脚被盖神经核(PPN)、三叉神经中间部位(IRT)等则表达较弱。结论:TASK-1 mRNA在脑干呼吸相关神经元上广泛表达,从解剖学分布上提示它可能以某种形式参与中枢的呼吸调控过程。
[Abstract]:Objective: Task-1 is a two-pore potassium leakage channel sensitive to pH in physiological range.The aim of this study was to investigate the distribution of TASK-1mRNA in the brain stem respiratory center.Methods: digoxin labeled oligonucleotide probes were used to detect the expression of TASK-1mRNA in brain stem respiratory related neurons by in situ hybridization with frozen brain stem sections.The neuronal types of TASK-1mRNA were identified by immunofluorescence histochemistry.Results there was a strong expression of% TASK-1 mRNA in the hypoglossal nucleus, the main upper airway motor nucleus. The expression of TASK-1 mRNA was found in the central chemosensory nucleus of ventral and dorsal medulla oblongata.The expression of PPN in the tegmental ganglion and IRTT in the middle of trigeminal nerve were weak.Conclusion the brain stem respiratory related neurons are widely expressed in the brain stem respiratory related neurons. The anatomical distribution suggests that the brain stem TASK-1 mRNA may be involved in the central respiratory regulation process in some way.
【作者单位】：北京大学第一医院:呼吸和危重症医学科;
【基金】：国家自然科学基金(81141002,81241004)
【分类号】：R56

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