益气化瘀解毒复方联合超低频电磁场处理水对内毒素诱发大鼠急性肺损伤的影响

发布时间：2018-04-14 03:22

本文选题：急性肺损伤 + 益气化瘀解毒复方　；参考：《中医杂志》2016年09期

【摘要】：目的探讨益气化瘀解毒复方联合超低频电磁场处理水(简称频谱水)对内毒素诱发大鼠急性肺损伤的影响及可能作用机制。方法 104只SD大鼠随机分为13组,每组8只。预防实验:预防频谱水组、预防频高组、预防频低组、预防普通水组、预防普高组、预防普低组分别给予相应水溶液2 ml灌胃,每日1次。14天后,除预防对照组外各组以5 mg/kg内毒素腹腔注射制备急性肺损伤模型。治疗实验:除对照组外,其余各组造模同预防实验,造模后频高组、普高组分别给予高剂量中药频谱水和普通水2 ml灌胃1次,血必净组予血必净注射液4.5 ml/kg腹腔注射1次。两个实验均进行大鼠肺大体病理、病理组织学光镜观察,并检测血清白细胞介素8(IL-8)、肿瘤坏死因子α(TNF-α)含量。结果预防实验中,与预防对照组比较,预防模型组IL-8、TNF-α水平升高(P0.05);与预防模型组比较,预防普低组、预防普高组、预防频谱水组、预防频低组、预防频高组IL-8、TNF-α水平均降低(P0.05);与预防频低组比较,预防频高组IL-8、TNF-α水平均降低(P0.05)。治疗实验中,与对照组比较,模型组IL-8、TNF-α水平明显升高(P0.05);与模型组比较,频高组、普高组、血必净组IL-8、TNF-α水平均降低(P0.05);与频高组比较,普高组IL-8、TNF-α水平均升高(P0.05)。结论益气化瘀解毒复方对内毒素致急性肺损伤具有一定的防治作用,可能与降低大鼠血清中IL-8、TNF-α水平有关,并且高剂量效果好;频谱水与中药复方合用有增强抗炎、抗肺损伤作用的趋势。
[Abstract]:Objective to investigate the effect and possible mechanism of Yiqi Huayu jiedu compound (YQHJD) combined with ultra-low frequency electromagnetic field (ultralow frequency electromagnetic field) treatment on endotoxin-induced acute lung injury in rats.Methods 104 SD rats were randomly divided into 13 groups with 8 rats in each group.Prevention experiment: the prevention spectrum water group, the prevention frequency high group, the prevention frequency low group, the prevention common water group, the prevention common water group, the prevention common low group, the prevention common low group were given the corresponding water solution 2ml by stomach respectively, after 1 day. 14 days later,The acute lung injury model was established by intraperitoneal injection of endotoxin for 5 mg/kg except the control group.Treatment experiment: except the control group, the other groups were in the same preventive experiment. The group of high frequency after model making and the group of Pugao were given 2 ml of traditional Chinese medicine frequency spectrum water and common water respectively, and the group of Xuebijing was injected intraperitoneally with Xuebijing injection 4.5 ml/kg.The contents of IL-8 and TNF- 伪 in serum were measured.Results compared with the control group, the level of IL-8 TNF- 伪 in the prevention model group was significantly higher than that in the prevention model group (P 0.05), and that in the prevention model group was higher than that in the prophylaxis model group, the prophylaxis group, the prophylaxis group, the prevention spectrum water group, and the prevention frequency low group.The level of IL-8 TNF- 伪 in high prevention frequency group was lower than that in low prevention frequency group, and the level of IL-8 TNF- 伪 in high prevention frequency group was lower than that in prevention frequency low frequency group.In the treatment experiment, compared with the control group, the level of IL-8 TNF- 伪 in the model group was significantly higher than that in the model group, and the levels of IL-8 TNF- 伪 in the high frequency group, Pugao group and Xuebijing group were all decreased compared with the control group, and the level of IL-8 TNF- 伪 in the high level group was higher than that in the high frequency group.Conclusion Yiqi Huayu jiedu compound has a certain preventive and therapeutic effect on acute lung injury induced by endotoxin, which may be related to the decrease of IL-8 TNF- 伪 level in serum of rats, and the high dose effect is good.Trend of anti-lung injury.
【作者单位】：北京中医药大学东直门医院;广东骏丰频谱股份有限公司生物频谱研究院;北京协和医学院;
【基金】：北京中医药大学2015年度校级自主课题(2015-JYBJSMS059) 广东骏丰频谱股份有限公司项目(HX-DZM-2015002)
【分类号】：R563

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