实验性矽肺大鼠中IP-10和TARC的表达及意义

发布时间：2018-04-18 03:12

本文选题：趋化性细胞因子 + IP-　；参考：《安徽医药》2012年06期

【摘要】：目的研究Th1相关趋化性细胞因子IP-10(IFN-γ-inducible protein,干扰素诱导蛋白10)和Th2相关趋化性细胞因子TARC(thymus and activation regulated chemokine,胸腺和活化调节趋化因子)在实验性矽肺大鼠染尘后不同时间点的表达水平及意义。方法将54只雄性SD大鼠随机分为模型组和对照组各27只。模型组采用非暴露式气管内一次性注入1 ml生理盐水二氧化硅悬液建立大鼠矽肺模型,对照组气管内注入1 ml灭菌生理盐水。分别在染尘后第1、7、14、21、28、35、77、106、126天处死两组大鼠各3只,结扎右肺,对左肺进行肺泡灌洗,收集其肺泡灌洗液并用ELISA法检测IP-10和TARC含量;免疫组化S-P法分析右肺组织中IP-10和TARC的表达情况。结果成功构建大鼠矽肺模型;模型组肺泡灌洗液中IP-10在第1、7、14、21天明显增多,与对照组相比差异有统计学意义(P0.05);TARC在第1、7、14、21、28、35天明显增多,与对照组相比差异有统计学意义(P0.05)。肺组织免疫组化结果用图像分析系统进行半定量分析,与对照组相比,染尘后第1、7、14、21天,模型组大鼠矽肺组织中IP-10表达差异有统计学意义(P0.05);染尘后第1、7、14、21、28、35天,模型组大鼠矽肺组织中TARC表达差异有统计学意义(P0.05)。结论 Th1和Th2相关趋化性细胞因子募集Th1及Th2细胞到肺组织中参与大鼠矽肺病变的免疫调节过程。
[Abstract]:Objective to study the expression level and significance of IFN- 纬 -inducible protein (IFN- 纬 -inducible protein) and TARC(thymus and activation regulated chemokine (thymus and activated regulatory chemokine) in experimental silicosis rats at different time points after exposure to dust.Methods 54 male Sprague-Dawley rats were randomly divided into two groups: the model group and the control group.In the model group, 1 ml saline silica suspension was injected into the trachea at one time, and 1 ml sterilized saline was injected into the trachea of the control group.Three rats in each group were killed on the 1st day after dust exposure, 3 rats in the right lung were ligated, the alveolar lavage fluid was collected and the contents of IP-10 and TARC were detected by ELISA method, and the expression of IP-10 and TARC in the right lung tissue was analyzed by immunohistochemical S-P method.Results the rat model of silicosis was successfully established, and the IP-10 in the alveolar lavage fluid of the model group was significantly increased on the 1st day, 1421 days, compared with the control group, the difference was statistically significant (P 0.05).Compared with the control group, the expression of IP-10 in silicosis tissue of the model group was significantly different from that of the control group on the 1st day after exposure to dust (P 0.05), and on the first day after exposure to dust, there was a significant difference in the expression of IP-10 between the two groups (P < 0.05), and the first day after exposure to dust, there was no significant difference in the expression of IP-10 between the two groups.The difference of TARC expression in silicosis in model group was statistically significant (P 0.05).Conclusion Th1 and Th2 related chemotactic cytokines are involved in the immunomodulation of rat silicosis by recruiting Th1 and Th2 cells into the lung tissue.
【作者单位】：安徽医科大学免疫学教研室;
【基金】：安徽省优秀青年科技基金(No 04043053) 教育部留学回国人员科研启动资金(No 2005383) 安徽省高校省级自然科学研究重点项目(No KJ2007A023)
【分类号】：R135.2

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