克拉霉素联合布地奈德对COPD大鼠糖皮质激素抵抗的影响

发布时间：2018-04-21 03:29

本文选题：克拉霉素 + 布地奈德　；参考：《郑州大学学报(医学版)》2017年03期

【摘要】：目的:探讨克拉霉素联合布地奈德对慢性阻塞性肺疾病(COPD)大鼠糖皮质激素抵抗的影响。方法:对大鼠进行COPD造模,然后分4组处理,COPD组、克拉霉素组、布地奈德组和联合干预组(克拉霉素+布地奈德组),每组10只;以10只未处理未造模大鼠作空白对照(空白组)。留取各组大鼠肺组织和血清,HE染色,光镜下观察肺组织病理学改变,采用ELISA法测定血清中TNF-α水平,采用RT-PCR及Western blot检测肺组织糖皮质激素受体α(GRα)及GRβ的表达。结果:与空白组相比,COPD组大鼠气管及支气管黏膜上皮细胞脱落,炎性细胞浸润,支气管平滑肌增厚,肺泡壁变薄,甚至破裂融合成肺大泡,血清TNF-α水平升高,提示造模成功。对COPD组、克拉霉素组、布地奈德组和联合干预组的分析表明,克拉霉素和布地奈德均可降低COPD大鼠血清TNF-α水平和肺组织GRβ mRNA和蛋白的表达水平,升高肺组织GRα mRNA和蛋白的表达水平,且二者联用有协同作用(P0.05)。结论:克拉霉素、布地奈德均可在不同程度上调节GRα/GRβ失衡,从而改善COPD大鼠糖皮质激素抵抗,二者联用抗炎效果更佳。
[Abstract]:Aim: to investigate the effect of clarithromycin combined with budesonide on glucocorticoid resistance in rats with chronic obstructive pulmonary disease (COPD). Methods: COPD models were made in rats and were divided into 4 groups: carramycin group, clarithromycin group, budesonide group and combined intervention group (clarithromycin budesonide group, 10 rats in each group) and 10 untreated untreated rats as blank control group (blank group). Lung tissue and serum of each group were stained with HE, the pathological changes of lung tissue were observed under light microscope, the level of TNF- 伪 in serum was measured by ELISA method, and the expression of glucocorticoid receptor 伪 -guar 伪 and gr 尾 in lung tissue was detected by RT-PCR and Western blot. Results: compared with the control group, the tracheal and bronchial mucosal epithelial cells in COPD group were shedded, inflammatory cells infiltrated, bronchial smooth muscle thickened, alveolar wall thinned, even ruptured and fused into alveoli, and the level of serum TNF- 伪 increased, which indicated that the model was successful. The analysis of COPD group, clarithromycin group, budesonide group and combined intervention group showed that clarithromycin and budesonide could decrease the level of serum TNF- 伪 and the expression of gr 尾 mRNA and protein in lung tissue of COPD rats. The expression of gr 伪 mRNA and protein in lung tissue was increased, and the combined use of gr 伪 and protein had synergistic effect on the expression of gr 伪 mRNA and protein. Conclusion: clarithromycin and budesonide can regulate the imbalance of gr 伪 / gr 尾 in different degree and improve glucocorticoid resistance in COPD rats.
【作者单位】：郑州大学第一附属医院呼吸内科;
【基金】：河南省科技厅攻关项目162102310196
【分类号】：R563.9

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