25羟维生素D3与抗菌肽在反复上呼吸道感染中的作用研究及相关性分析

发布时间：2018-04-22 17:12

本文选题：反复上呼吸道感染 + 羟维生素D　；参考：《重庆医学》2016年29期

【摘要】：目的探讨25羟维生素D3与抗菌肽在反复上呼吸道感染的作用。方法选择连续3年就诊的上呼吸道感染患者,其中,1年上呼吸道感染次数大于或等于2次患者46例,男20例,女26例,平均(32.35±9.10)岁;选择15例人作为对照组,平均(31.35±10.20)岁,对照组在年龄上与上呼吸道感染患者差异无统计学意义(P0.05)。采用酶联免疫吸附测定(ELISA)检测外周血血清中25羟维生素D3水平和诱导痰上清液抗菌肽LL-37水平并做相关性分析。用人气道上皮细胞株分析25羟维生素D3对抗菌肽LL-37水平影响。结果反复上呼吸道感染患者外周血血清25羟维生素D3水平低于对照组(P0.05),并且反复上呼吸道感染患者诱导痰上清液人抗菌肽LL-37水平低于对照组(P0.05)。上呼吸道感染平均次数与上呼吸道感染患者外周血25羟维生素D3呈负相关(r=-0.54,P0.05),上呼吸道感染平均次数与诱导痰上清液抗菌肽LL-37水平呈负相关(r=-0.65,P0.05),上呼吸道感染患者外周血25羟维生素D3和诱导谈上清液抗菌肽LL-37水平呈正相关(r=0.59,P0.05)。体外细胞实验显示1,25羟维生素D3能上调上皮细胞抗菌肽LL-37表达。结论 25羟维生素D3的缺乏可能是上呼吸道反复感染患抗菌肽LL-37的表达下调的重要原因。
[Abstract]:Objective to investigate the effect of 25 hydroxyvitamin D3 and antibacterial peptide in recurrent upper respiratory tract infection. Methods the patients with upper respiratory tract infection for 3 years were selected, among them, 46 cases of upper respiratory tract infection more than or equal to 2 times in 1 years, 20 men and 26 women, with an average of (32.35 + 9.10) years, and 15 people as the control group, average (31.35 + 10.20) years of age. There was no significant difference between the age and the upper respiratory tract infection (P0.05). Enzyme linked immunosorbent assay (ELISA) was used to detect the level of 25 hydroxyvitamin D3 in the serum of the peripheral blood and the level of antibacterial peptide LL-37 in the induced sputum supernatant and to make a correlation analysis. The level of 25 hydroxyvitamin D3 was analyzed by the human airway epithelial cell line. Results the level of 25 hydroxyvitamin D3 in peripheral blood serum of patients with recurrent upper respiratory tract infection was lower than that of the control group (P0.05), and the level of LL-37 in the induced sputum supernatant was lower than that of the control group (P0.05). The average number of upper respiratory tract infection was negatively correlated with the peripheral blood 25 hydroxyvitamin D3 (r=-0) in upper respiratory tract infection (r=-0 .54, P0.05), the average frequency of upper respiratory tract infection was negatively correlated with the level of antibacterial peptide LL-37 of the induced sputum supernatant (r=-0.65, P0.05). 25 hydroxyvitamin D3 in peripheral blood of patients with upper respiratory tract was positively correlated with the level of antibacterial peptide LL-37 in the induced supernatant (r=0.59, P0.05). In vitro cell experiment showed that 1,25 hydroxyvitamin D3 could up regulate the antibacterial peptide of epithelial cells. LL-37 expression. Conclusion the lack of 25 hydroxyvitamin D3 may be an important reason for the down-regulation of antimicrobial peptide LL-37 in patients with repeated respiratory tract infections.

【作者单位】：西南医科大学卫生科;西南医科大学附属医院炎症与变态反应实验室;西南医科大学附属医院呼吸内科;
【基金】：国家自然科学基金资助项目(81170032)
【分类号】：R56

【相似文献】