肺结核肺组织干扰素-γ表达与其病理损伤间的关系研究

发布时间：2018-04-23 00:25

本文选题：肺结核 + 干扰素-γ　；参考：《实用医学杂志》2014年10期

【摘要】：目的:研究细胞因子干扰素-γ(IFN-γ)在肺组织中的表达变化与肺组织病理损伤间的相互关系,为肺结核的防治提供参考。方法:毒性结核分枝杆菌H37Rv尾静脉注射BALB/c小鼠建立肺结核动物模型,分别于感染后0(未感染)、2、4、6、8周末,进行肺组织细菌载荷量计数及组织病理观察,免疫组织化学染色检测IFN-γ、IL-2及TNF-α在肺组织的表达。结果:MTB感染后2周末,IFN-γ、IL-2及TNF-α的阳性细胞百分比较0周时均显著升高,感染后6周末(9.25%)至8周末(5.67%),IFN-γ阳性细胞百分比显著下降(P0.01)。而该时期TNF-α及IL-2的表达均有下降趋势,但与感染后2周或4周时无明显差异(P0.05)。感染后8周末,肺组织细菌载荷量(8.43)及肺组织病理分数(17)较感染6周时均显著升高(P0.01)。结论:MTB感染早期,肺组织IFN-γ、TNF-α及IL-2均呈较高表达,对肺组织具有较强的保护作用。MTB持续感染期,IFN-γ表达量下降明显加剧肺组织损伤。
[Abstract]:Objective: to study the relationship between the expression of cytokine interferon- 纬 (IFN- 纬) in lung tissue and the pathological injury of lung tissue, so as to provide reference for the prevention and treatment of pulmonary tuberculosis. Methods: the pulmonary tuberculosis animal model was established by injecting H37Rv tail vein into BALB/c mice with Mycobacterium tuberculosis. The count of bacterial load and histopathology of pulmonary tissue were observed at the end of 8 weeks after infection. The expression of IL-2 and TNF- 伪 in lung tissue was detected by immunohistochemical staining. Results the percentage of IFN- 纬 IL-2 and TNF- 伪 positive cells increased significantly at the end of 2 weeks after infection, and the percentage of IFN- 纬 positive cells decreased significantly at the end of 6 weeks after infection and 9.25% at the end of 6 weeks after infection) and the percentage of IFN- 纬 positive cells decreased significantly at the end of 8 weeks after infection. The expression of TNF- 伪 and IL-2 decreased in this period, but there was no significant difference between TNF- 伪 and IL-2 at 2 or 4 weeks after infection. At the end of 8 weeks after infection, the amount of bacterial load in lung tissue (8.43) and the pathological score of lung tissue (17) were significantly higher than those at 6 weeks after infection (P 0.01). Conclusion both IFN- 纬 TNF- 伪 and IL-2 are highly expressed in lung tissue at the early stage of infection. The decrease of IFN- 纬 expression in lung tissue during the period of persistent infection of TNF- 伪 and TNF- 伪 has a strong protective effect on lung tissue. The decrease of IFN- 纬 expression in lung tissue obviously exacerbates the injury of lung tissue.
【作者单位】：昆明理工大学医学院人体解剖与组织胚胎学教研室;
【基金】：云南省教育厅科研基金(编号:2012C069)
【分类号】：R521

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