COPD患者血清网膜素-1水平变化与TNF-a、VEGF及ET-1相关性分析

发布时间：2018-04-24 05:19

本文选题：慢性阻塞性肺疾病 + 网膜素　；参考：《遵义医学院》2017年硕士论文

【摘要】：目的:观察慢性阻塞性肺疾病急性加重期、稳定期患者及健康成人之间血清网膜素-1(omentin)水平以及与TNF-a、VEGF及ET-1的相关性。方法:选取2015年7月至2016年7月在深圳市龙岗中心医院呼吸内科的30例COPD急性期住院病人及30例门诊随诊的稳定期病人,对照组为30例肺功能正常的同龄健康体检者。分别记录受试者的年龄、性别,完善肺功能检查,抽取受试者外周静脉血,采用酶联免疫吸附法(ELISA)检测网膜素-1、TNF-a、VEGF及ET-1在血清中的水平,并使用SPSS软件比较各组间指标的差异性,并进一步分析网膜素-1与TNF-a、VEGF及ET-1的相关性。结果:1、急性期、稳定期患者与健康对照组在性别、年龄等一般资料之间无显著性差异,而肺功能FEV1的值下降具有统计学差异。2、急性期组血清网膜素-1水平为(35.10±4.83)ng·ml-1,稳定期组血清网膜素-1水平为(40.13±4.34)ng·ml-1,健康对照组血清网膜素-1水平为(51.90±5.73)ng·ml-1,三组间互有差异。3、急性期组血清TNF-a水平为(570.84±75.71)pg·ml-1,稳定期组TNF-a血清水平为(370.63±61.80)pg·ml-1,健康对照组血清TNF-a水平为(321.08±58.53)pg·ml-1,三组间互有差异。4、急性期组血清VEGF水平(193.81±18.53)pg·ml-1,稳定期组血清VEGF水平为(162.94±14.90)pg·ml-1,健康对照组血清VEGF水平为(151.90±18.29)pg·ml-1,三组间互有差异。5、急性期组血清ET-1水平(134.00±15.47)pg·ml-1,稳定期组血清ET-1水平(100.70±11.69)pg·ml-1,健康对照组血清ET-1水平(92.14±15.23)pg·ml-1,健康对照组与稳定期组间ET-1的平均水平差异无统计学差异,其余组间差异均有统计学差异。6、急性期组网膜素-1与TNF-a呈负相关(r=-0.633),与VEGF、ET-1无明显相关,稳定期组网膜素-1与TNF-a呈负相关(r=-0.614),与VEGF、ET-1无明显相关。结论:(1)COPD患者血清网膜素-1水平降低且与TNF-a水平升高成负相关,可能参与了慢性阻塞性肺疾病的慢性炎症过程;(2)COPD患者血清网膜素-1水平降低及VEGF、ET-1水平升高可能通过不同机制参与肺部血管收缩和内皮细胞功能紊乱。
[Abstract]:Aim: to investigate the relationship between serum omentin-1 (omentin) levels and TNF-a ET-1 levels in patients with acute exacerbation of chronic obstructive pulmonary disease (COPD), stable patients and healthy adults. Methods: from July 2015 to July 2016, 30 COPD patients in the Department of Respiratory Medicine, Shenzhen Longgang Central Hospital, and 30 stable patients in out-patient service were selected. The control group consisted of 30 healthy people of the same age with normal lung function. The age, sex and lung function of the subjects were recorded respectively. Peripheral venous blood was extracted, and the levels of omentin-1 TNF-a ET-1 in serum were detected by enzyme linked immunosorbent assay (Elisa). SPSS software was used to compare the difference of the indexes among the groups. The relationship between omentin-1 and ET-1 was analyzed. Results there was no significant difference in sex, age and other general data between the patients in the acute stage, the stable stage and the healthy control group. The level of omentin-1 was 35.10 卤4.83)ng ml-1 in acute group, 40.13 卤4.34)ng ml-1 in stable group, and 51.90 卤5.73)ng ml-1 in healthy control group. The serum TNF-a level of phase group was 570.84 卤75.71)pg ml-1, the serum level of TNF-a in stable phase group was 370.63 卤61.80)pg ml-1, the serum TNF-a level of healthy control group was 321.08 卤58.53)pg ml-1. There was a significant difference among the three groups. The serum VEGF level of acute phase group was 193.81 卤18.53)pg ml-1, the serum VEGF level of stable phase group was 162.94 卤14.90)pg ml-1, and that of healthy control group was VEGF water level. The serum ET-1 level was 134.00 卤15.47)pg ml-1 in acute group, 100.70 卤11.69)pg ml-1 in stable phase group, 92.14 卤15.23)pg ml-1 level in healthy control group. There was no significant difference in average ET-1 level between healthy control group and stable group. There were significant differences between the other groups. There was a negative correlation between reticulin-1 and TNF-a in acute phase, but no significant correlation with VEGF ET-1, but a negative correlation between reticulin-1 and TNF-a in stable phase, but no significant correlation with VEGFET-1. Conclusion the level of omentin-1 in the serum of patients with TNF-a was decreased and negatively correlated with the level of TNF-a. The decrease of serum omentin-1 level and the increase of VEGF ET-1 level may be involved in pulmonary vasoconstriction and endothelial cell dysfunction through different mechanisms in COPD patients who may be involved in the chronic inflammatory process of chronic obstructive pulmonary disease (COPD).
【学位授予单位】：遵义医学院
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R563.9

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