丙酮酸乙酯对TDI诱导的哮喘小鼠气道上皮细胞黏附连接蛋白E-cadherin的影响

发布时间：2018-04-27 01:09

本文选题：丙酮酸乙酯 + TDI哮喘　；参考：《实用医学杂志》2014年22期

【摘要】：目的:观察丙酮酸乙酯(EP)对甲苯二异氰酸酯(TDI)诱导的哮喘小鼠气道上皮细胞黏附连接蛋白E-cadherin表达的影响。方法:BALB/c小鼠30只,随机分为3组。哮喘组第1天、第8天予0.3%TDI耳背致敏,第15、18、21天予3%TDI激发,每次激发前1 h按100 mg/kg腹腔注射生理盐水。对照组第1天、第8天予AOO(丙酮和橄榄油)致敏,余同哮喘组。EP治疗组,同哮喘组,但在激发前1 h按100 mg/kg腹腔注射EP。第22天检测各组小鼠的气道高反应性、淋巴上清IL-4、IFN-γ及血清Ig E,用免疫组化及WB测定肺组织E-cadherin。结果:哮喘组气道高反应性、淋巴上清IL-4、IFN-γ及血清Ig E均高于对照组,EP干预后显著降低。免疫组化及免疫印迹结果示,对照组E-cadherin均匀致密分布于气道上皮细胞连接处,哮喘组Ecadherin分布混乱、减少,EP干预能显著减少E-cadherin的破坏。结论:EP可以减少TDI诱导的哮喘小鼠气道上皮细胞E-cadherin的破坏。
[Abstract]:Objective: To observe the effect of ethyl pyruvate (EP) on the expression of adhesion connexin E-cadherin in airway epithelial cells in asthmatic mice induced by toluene diisocyanate (TDI). Methods: 30 BALB/c mice were randomly divided into 3 groups. The asthma group was first days, eighth days to 0.3%TDI ear sensitization, 3%TDI excitation on the day 15,18,21, and 100 mg/kg celiac before each stimulation of 1 h. The control group was injected with AOO (acetone and olive oil) on the eighth day for first days. The control group was treated with asthma group.EP and asthma group, but the airway hyperresponsiveness of each group was detected by 100 mg/kg intraperitoneal injection of EP. twenty-second days before the stimulation of 1 h. Lymphatic supernatant IL-4, IFN- gamma and Ig E in blood, immunohistochemistry and WB were used to determine the E-cadherin. results of lung tissue. The airway hyperresponsiveness, IL-4, IFN- gamma and serum Ig E in the asthmatic group were all higher than those in the control group, and the results of EP intervention were significantly lower. The immunohistochemical and Western blot results showed that the E-cadherin in the control group was uniformly distributed at the junction of the airway epithelial cells, the Ecadherin distribution in the asthmatic group was chaotic and decreased, and the EP intervention could significantly reduce the disruption of E-cadherin. Conclusion: EP can reduce the destruction of E-cadherin in airway epithelial cells of TDI induced asthmatic mice.

【作者单位】：南方医科大学南方医院呼吸与危重症医学科慢性气道疾病实验室;
【基金】：国家自然科学基金(编号:81270087,81270089) 国家重点基础研究发展计划(“973计划”)(编号:2012CB518200) 广东省教育部产学研结合项目(编号:2012B091100153) 广东省科技计划项目(编号:2011B031800245)
【分类号】：R562.25

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