肿瘤坏死因子对急性肺损伤动物模型外周血骨髓间充质干细胞数量的影响

发布时间：2018-05-01 12:18

本文选题：急性肺损伤 + 骨髓间充质干细胞　；参考：《中国现代医学杂志》2014年05期

【摘要】：目的观察肿瘤坏死因子(TNF-α)对急性肺损伤动物模型外周血骨髓间充质干细胞(MSCs)数量的影响。方法 SD大鼠分为对照组、模型组和干预组。对照组给予生理盐水气道滴入;模型组给予内毒素(LP S 5 mg/kg)气道内滴入;干预组气道内滴入LPS同时英夫利昔(10 mg/mL)腹腔注射5 mg/kg。5 h后处死动物取外周血,检测TNF-α含量及成纤维细胞集落形成单位(CFU-F)数。成骨、成脂诱导检测其分化特性。结果模型组大鼠外周血TNF-α水平较对照组显著升高(354.0±82.4 vs 38.0±21.6,P0.05),而较干预组显著降低(206.5±64.1 vs 354.0±82.4,P0.05)。模型组大鼠外周血中培养出的CFU-Fs明显高于对照组(3.60±1.47vs 1.40±2.83,P0.05)及干预组(3.60±1.47 vs 1.70±1.72,P0.05);外周血骨髓MSCs成骨、成脂诱导后染色均阳性。结论急性肺损伤时,外周血中增加的TNF-α含量对骨髓MSCs动员及归巢均有促进作用。
[Abstract]:Objective to observe the effect of TNF- 伪 on the number of bone marrow mesenchymal stem cells (MSCs) in acute lung injury model. Methods SD rats were divided into control group, model group and intervention group. The control group was given normal saline, the model group was given endotoxin LP S 5 mg / kg, the intervention group was injected with LPS and Inflexixide 10 mg / mL intraperitoneally for 5 mg/kg.5, and the animals were killed to collect the peripheral blood after 5 mg/kg.5. The content of TNF- 伪 and the number of fibroblast colony forming units (CFU-F) were measured. Osteogenesis and adipogenic induction were used to detect its differentiation characteristics. Results compared with the control group, the level of TNF- 伪 in the model group was significantly higher than that in the control group (354.0 卤82.4 vs 38.0 卤21.6P0.05), while the level of TNF- 伪 was significantly lower than that in the intervention group (206.5 卤64.1 vs 354.0 卤82.4 P0.05). The CFU-Fs cultured in the peripheral blood of the model group was significantly higher than that of the control group (3.60 卤1.47vs 1.40 卤2.83 P0.05) and the intervention group (3.60 卤1.47 vs 1.70 卤1.72 P0.05), and the MSCs of peripheral blood was positive in osteogenesis and lipogenic induction. Conclusion during acute lung injury, the increased TNF- 伪 content in peripheral blood can promote bone marrow MSCs mobilization and homing.
【作者单位】：西安交通大学医学院第二附属医院呼吸内科;
【基金】：2010年陕西省卫生厅科研资助项目(No:2010D38)
【分类号】：R563.8

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