规范化特殊染色技术对肺真菌感染性疾病病理诊断的意义

发布时间：2018-05-10 20:46

本文选题：肺疾病 + 全自动特殊染色　；参考：《临床与实验病理学杂志》2017年06期

【摘要】：目的探讨规范化特殊染色技术在肺真菌感染性疾病病理诊断中应用的意义。方法选取2011年9月~2016年3月北京朝阳医院最终病理诊断为肺真菌感染性疾病104例,行HE染色、HE染色联合传统手工特殊染色法染过碘酸雪夫氏染色(PAS)及六胺银和HE染色联合全自动特殊染色法染PAS及六胺银,将两种特染技术规范化比较,镜下观察分析结果(均为初次染色结果,不包括复染结果),以最终病理诊断结果为标准,与临床初诊结果比较。结果肺真菌感染性疾病确诊率和真菌分型率,从低到高顺序一致,为临床初步诊断(29.8%和19.2%)、HE染色法(32.7%和32.7%)、HE染色联合传统手工特殊染色法染PAS和六胺银(90.4%和87.5%)、HE染色联合全自动特殊染色法染PAS和六胺银(98.1%和94.2%)。这4种方法从两方面比较差异有统计学意义(P0.01,P0.01),第4种方法与前两种比较方法差异有统计学意义(P0.01,P0.01),与第3种方法比较确诊率差异有统计学意义(P0.05),分型率差异无统计学意义(P0.05)。但全自动特殊染色法染PAS及六胺银操作步骤更简单,化学试剂规范化,无人工和环境因素影响,耗时短,脱片和复染张数更少。结论 HE染色法联合全自动特殊染色法染PAS和六胺银更加规范化,有助于提高肺真菌感染性疾病的确诊率和真菌分型率。
[Abstract]:Objective to explore the significance of standardized special staining technique in pathological diagnosis of pulmonary fungal infections. Methods 104 cases of pulmonary fungal infection were selected from September 2011 to March 2016 in Beijing Chaoyang Hospital. He and HE staining combined with traditional manual special staining were used to stain Pass and HE staining combined with automatic special staining for PAS and silver hexamine. The two special staining techniques were compared with each other. The results of microscopic observation and analysis (all of them were primary staining results, excluding the results of repeated staining) were compared with the results of clinical initial diagnosis according to the criteria of final pathological diagnosis. Results the rates of diagnosis and classification of pulmonary fungal infectious diseases were consistent from low to high. To make a preliminary diagnosis of 29.8% and 19.2% of them by HE staining, 32.7% and 32.7% of them were stained with PAS, 90.4% and 87.5% of them by traditional manual special staining, and 98.1% and 94.2% of them were stained with PAS and 94.1% and 94.2% respectively by fully automatic special staining. There were significant differences between the four methods in two aspects, the fourth method had statistical significance compared with the former two methods, and the difference between the four methods and the third method had statistical significance (P0.05A), but there was no significant difference in the classification rate between the four methods and the former two methods (P0.055.There was no significant difference in the classification rate between the four methods and the third method). However, the procedure of automatic special staining for PAS and hexamethylamine silver is simpler, the chemical reagent is standardized, the time is short, the number of strip and multiple dye is less, the time is short without the influence of artificial and environmental factors. Conclusion HE staining combined with automatic special staining for PAS and silver hexamine is more standardized, which is helpful to improve the rate of diagnosis and classification of pulmonary fungal infections.
【作者单位】：首都医科大学附属朝阳医院病理科;
【分类号】：R519

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