不同浓度TGF-β1对肺泡II型细胞系MLE-12表型及功能的影响

发布时间：2018-05-13 14:23

本文选题：TGF-β + 肺泡II型细胞　；参考：《中国细胞生物学学报》2014年04期

【摘要】：探讨不同浓度及不同时间点TGF-β1对肺泡II型上皮细胞(AECII)表型及功能的影响。小鼠肺泡II型细胞系MLE-12,随机分为:对照组(0 ng/mL)、低浓度组(0.1 ng/mL)、中浓度组(1 ng/mL)和高浓度组(10 ng/mL)。应用细胞免疫荧光双标法及荧光定量PCR法观察各组12,24,48,72 h细胞形态变化、AECII标记(肺表面活性物质蛋白B,SP-B)及成纤维细胞标记(成纤维细胞特异性蛋白1,FSP-1)蛋白及mRNA的表达情况。结果表明,随着TGF-β1干预时间的延长及浓度的升高,AECII逐渐由鹅卵石样变成纺锤体形状,获得成纤维细胞样外观。蛋白水平,AECII标记SP-B表达逐渐减弱,成纤维细胞标记FSP-1表达逐渐增强,48 h中浓度组及24 h高浓度组两者可见明显的共表达,同时,其SP-B mRNA表达较同时间对照组下调,而FSP1 mRNA表达较同时间对照组上调。低浓度组各时间点上述表现不明显。TGF-β1促使AECII向成纤维细胞转化(EMT),且具有时间及浓度依赖性。
[Abstract]:To investigate the effects of TGF- 尾 1 at different concentrations and at different time points on the phenotype and function of alveolar type II epithelial cells (AECIIs). Mouse alveolar type II cell line MLE-12 was randomly divided into three groups: control group (0 ng / mL), low concentration group (0.1 ng / mL), middle concentration group (1 ng / mL) and high concentration group (10 ng / mL). The expression of AECII (pulmonary surfactant protein), fibroblast specific protein (FSP-1) and the expression of AECII (pulmonary surfactant protein) and fibroblast specific protein (FSP-1) were observed by immunofluorescence double labeling method and fluorescence quantitative PCR method. The results showed that AECII gradually changed from pebble to spindle shape with the prolongation of TGF- 尾 _ 1 intervention time and the increase of TGF- 尾 _ 1 concentration, and the appearance of fibroblast was obtained. The expression of SP-B labeled by AECII was gradually decreased, and the expression of FSP-1 in fibroblasts was gradually increased. The co-expression of SP-B mRNA was observed in the groups of 48 h and 24 h. Meanwhile, the expression of SP-B mRNA was down-regulated compared with that of the control group at the same time. The expression of FSP1 mRNA was higher than that of the control group at the same time. In the low concentration group, the above performance was not obvious at each time point. TGF- 尾 1 promoted the transformation of AECII into fibroblasts in a time-and concentration-dependent manner.
【作者单位】：重庆医科大学附属儿童医院呼吸中心儿科学重庆市重点实验室重庆市儿童发育重大疾病诊治与预防国际合作基地儿童发育疾病研究省部共建教育部重点实验室;
【基金】：国家自然科学基金(批准号:30770950)资助的课题~~
【分类号】：R563

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