部分致病菌感染肺组织产生的特征性挥发性有机物的研究

发布时间：2018-05-25 07:54

本文选题：大肠杆菌 + 金黄色葡萄球菌　；参考：《浙江大学》2013年硕士论文

【摘要】：目的：通过检测部分常见致病菌感染的肺组织代谢过程中产生的挥发性有机物(volatile organic compounds, VOCs),筛选出各菌株的特征性VOCs,为呼吸气体检测在肺部感染性疾病的早期诊断中的应用提供实验依据。方法：建立致病菌感染的人体外肺组织模型及致病菌感染的动物模型,利用固相微萃取联合气相色谱质谱仪连用技术(solid-phase microextraction and gas chromatography mass spectroscopy, SPME/GC-MS),通过对大肠杆菌、金黄色葡萄球菌及铜绿假单胞菌感染的肺组织所产生的呼吸气体的检测,筛选出这三种致病菌的特征性VOCs, 结果：在致病菌感染人体外肺组织模型中,大肠杆菌感染产生的VOCs中可以检测到十二烷(C12H26)、间-(1,1-二甲基-乙基)-苯(C14H22).十六醇(C16H34O),金黄色葡萄球菌感染产生的VOCs中可以检测到C12H26、C14H22、2.5-二(1.1-二甲基-乙基)-苯酚(C14H22O),铜绿假单胞菌感染产生的VOCs中可以检测到C12H26、C14H22、2,4,6-三(1,1-二甲基-乙基)-苯酚(C18H30O)。在致病菌感染的动物模型(体内培养模型)中,大肠杆菌感染产生的VOCs中可以检测到特异性标志物C16H34O,金黄色葡萄球菌感染产生的VOCs中可以检测到特异性标志物C14H22O,铜绿假单胞菌感染产生的VOCs中可以检测到特异性标志物C18H30O。结论：通过两种感染模型VOCs检测结果可知,大肠杆菌、金黄色葡萄球菌、铜绿假单胞菌感染的肺组织在代谢过程中可产生特征性VOCs,其中十六醇(C16H34O)是大肠杆菌感染肺部组织产生的特征性标志物,2,5-二(1,1-二甲基-乙基)-苯酚(C14H22O)是金黄色葡萄球菌感染产生的特征性标志物,2,4,6-三(1,1-二甲基-乙基)-苯酚(C18H30O)是铜绿假单胞菌感染产生的特征性标志物。
[Abstract]:Purpose :

The volatile organic compounds ( VOCs ) generated during the metabolic process of lung tissues infected by common pathogenic bacteria were detected , and the characteristic VOCs of each strain were screened , and the experimental basis was provided for the application of respiratory gas detection in the early diagnosis of lung infectious diseases .

Method :

By using solid - phase extraction and gas chromatography mass spectroscopy ( GC - MS ) , the characteristic VOCs of these three pathogenic bacteria were selected by means of solid - phase extraction and gas chromatography mass spectroscopy ( GC - MS ) , and the respiratory gases produced by lung tissues infected by E . coli , Staphylococcus aureus and Pseudomonas aeruginosa were selected by solid - phase extraction and gas chromatography mass spectroscopy ( GC - MS ) .

Results :

C12H26 , C14H22O , C14H22O , C14H22O , C14H22O , C14H22O , C14H22O , C14H22O , C14H22O , C14H22O , C14H22O , C14H22O , C14H22O , C14H22O , C14H22O , C14H22O , C14H22O and C14H22O could be detected in VOCs .

Conclusion :

It can be seen from the results of the two kinds of infection models that the lung tissues infected by E . coli , S . aureus and Pseudomonas aeruginosa can produce characteristic VOCs during metabolism , wherein cetyl alcohol ( C16H34O ) is a characteristic marker of E . coli infection of lung tissue , and 2,5 - bis ( 1,1 - dimethyl - ethyl ) - phenol ( C14H22O ) is a characteristic marker of Staphylococcus aureus infection , and 2,4,6 - tris ( 1,1 - dimethyl - ethyl ) - phenol ( C18 H30O ) is a characteristic marker for Pseudomonas aeruginosa infection .
【学位授予单位】：浙江大学
【学位级别】：硕士
【学位授予年份】：2013
【分类号】：R563.1

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