改良兔体外循环急性肺损伤模型的建立

发布时间：2018-05-26 13:11

本文选题：体外循环 + 急性肺损伤　；参考：《南方医科大学学报》2017年06期

【摘要】：目的探讨改良经主动脉、右心房插管,自体血预充的兔体外循环(CPB)急性肺损伤模型,及外周血肿瘤坏死因子α水平变化。方法随机选择10只健康成年雄性新西兰兔建立体外循环模型。待心脏复跳稳定,终止体外循环后,兔存活4 h即判定为模型制作成功。监测生命体征,分别记录在麻醉后(T_1)、在体外循环转流前(T_2),阻断主肺动脉后15 min时(T_3),主肺动脉的重新开放灌流后(T_4),体外循环结束后1 h(T_5)、4 h(T_6)的生命体征值,与T2、T_4、T_6时刻采集动脉血进行血气分析,采用酶联免疫吸附(ELISA)法检测T2、T_5、T_6时刻外周血中肿瘤坏死因子α(TNF-α)水平。结果通过对10只兔的实验,成功改良了兔CPB急性肺损伤模型。术中平均动脉压维持55 mm Hg以上,体外转流前后红细胞压积显著下降(T230.18±2.88%,T_417.73±1.95%,P0.05),血浆乳酸Lac浓度逐步升高,开放主肺动脉时明显增加(T23.65±1.13 mmol/L,T_49.36±1.28 mmol/L,P0.05)。T_6氧合指数(PaO_2/FiO_2)比T2显著下降(T2468.36±56.28 mm Hg,T_6281.64±55.76 mm Hg,P0.05),血清中TNF-α水平显著升高(P0.05),肺间质水肿显著,炎性细胞浸润增加。结论本实验改良的兔CPB急性肺损伤模型稳定可靠,可为研究体外循环引起的急性肺损伤提供稳定可靠的研究基础。
[Abstract]:Objective to investigate the changes of tumor necrosis factor 伪 (TNF 伪) in peripheral blood of modified rabbit model of acute lung injury induced by aortic catheterization, right atrial intubation and autologous blood prefilled with cardiopulmonary bypass (CPB). Methods 10 healthy adult New Zealand rabbits were randomly selected to establish cardiopulmonary bypass (CPB) model. After the cardiopulmonary bypass (CPB) was terminated, the rabbit survived for 4 hours and the model was established successfully. To monitor vital signs and record the vital signs of T _ 2 / T _ 2 before cardiopulmonary bypass (CPB), 15 min after CPB, 15 min after obstruction of the main pulmonary artery, 1 h after the reopening of the main pulmonary artery, and 1 h after cardiopulmonary bypass (CPB), to record the vital signs of T _ 1 / T _ 1, T _ 2 / T _ 2, T _ 3 / T _ 3, and T _ 4 / T _ 4 after reopening and perfusing the main pulmonary artery, and 1 h after cardiopulmonary bypass, Arterial blood was collected from T _ 2 / T _ 4 / T _ T _ 6 for blood gas analysis and the level of TNF- 伪 in peripheral blood of T _ (2) T _ (5) and T _ (6) was detected by Elisa Elisa. Results the acute lung injury model of CPB was successfully improved in 10 rabbits. During the operation, the mean arterial pressure was maintained above 55 mm Hg, and the hematocrit decreased significantly before and after external bypass, T230.18 卤2.88 and T _ (417.73 卤1.95) P 0.05, and the plasma lactate Lac concentration increased gradually. There was a significant increase in T23.65 卤1.13 mmol 路L ~ (-1) T _ T _ (49.36 卤1.28) mmol 路L ~ (-1) mol 路L ~ (-1) 路T _ (6) oxygenation index P _ (2 / FiO _ 2) compared with T _ 2. Compared with T _ 2, T _ (2468.36) 卤56.28 mm Hgg / T _ T _ T _ (6281.64 卤55.76 mm) HgT _ (0.05), serum TNF- 伪 level, pulmonary edema and inflammatory cell infiltration were significantly increased. Conclusion the modified CPB model of acute lung injury in rabbits is stable and reliable, which can provide a stable and reliable basis for the study of acute lung injury induced by cardiopulmonary bypass.
【作者单位】：南方医科大学南方医院麻醉科;陆军总医院附属八一儿童医院儿童SPICU;
【基金】：国家自然科学青年科学基金(81400309)~~
【分类号】：R-332;R563.8

【相似文献】