地塞米松对肺炎支原体感染大鼠血清及肺泡灌洗液中IL-12、IL-13的影响

发布时间：2018-05-27 12:30

本文选题：地塞米松 + 支原体肺炎　；参考：《临床儿科杂志》2014年03期

【摘要】：目的研究地塞米松对肺炎支原体(MP)感染大鼠血清及肺泡灌洗液(BALF)中IL-12、IL-13的影响,探讨其治疗MP肺炎的作用机制。方法 100只SD大鼠随机分为4组:阿奇霉素组、地塞米松组、联合干预组和模型对照组,每组各25只。各组大鼠均予MP菌液(1×106CFU/ml)缓慢滴入大鼠鼻腔,共4 d,在最后1次接种后第2天开始干预。采用ELISA方法监测干预后第3、5、8天大鼠血清及BALF液IL-12、IL-13水平,并对肺组织炎症浸润程度进行评分。结果在干预第3、5、8天,阿奇霉素组、地塞米松组、联合干预组及模型对照组肺组织病理评分比较差异均有统计学意义(F=8.71~20.11,P均0.01),其中均以联合干预组评分最低。在干预第3、5、8天,四组间血清及BALF液中IL-12、IL-13水平比较差异均有统计学意义(F=9.52~143.47,P均0.01);其中干预第3天,联合干预组血清IL-12高于阿奇霉素组,而血清及BALF液IL-13均低于阿奇霉素组,差异均有统计学意义(P均0.05)。结论地塞米松可能协同阿奇霉素通过促进大鼠体内IL-12分泌和抑制IL-13表达,减轻肺组织炎症。
[Abstract]:Objective to study the effect of dexamethasone on IL-12 IL-13 in serum and alveolar lavage fluid (BALF) of rats infected with MP, and to explore the mechanism of the treatment of MP pneumonia. Methods 100 SD rats were randomly divided into 4 groups: azithromycin group, dexamethasone group, combined intervention group and model control group with 25 rats in each group. All the rats in each group were treated with 1 脳 10 6 CFU / ml MP bacteria solution, which was slowly dripped into the nasal cavity of the rats for 4 days, and the intervention began on the second day after the last inoculation. The levels of IL-12 IL-13 in serum and BALF fluid of rats were monitored by ELISA method on the 3rd day and 8th day after intervention, and the degree of inflammatory infiltration in lung tissue was evaluated. Results on the 5th day of intervention, the pathological scores of lung tissue in azithromycin group, dexamethasone group, combined intervention group and model control group were significantly different. On the 3rd day of intervention, the levels of IL-12 and IL-13 in serum and BALF solution were significantly different among the four groups (P < 0.01), and on the third day of intervention, the serum IL-12 of the combined intervention group was higher than that of the azithromycin group, while the serum and BALF solution IL-13 levels were lower than those of the azithromycin group. The difference was statistically significant (P < 0.05). Conclusion Dexamethasone combined with azithromycin may reduce lung inflammation by promoting IL-12 secretion and inhibiting IL-13 expression in rats.
【作者单位】：天津中医药大学;天津市南开医院儿科;
【基金】：天津市卫生局科技基金研究项目(No.09Ky21)
【分类号】：R563.1

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