补肺益肾方联合舒肺贴对慢性阻塞性肺疾病大鼠T淋巴细胞亚群的影响

发布时间：2018-05-31 14:12

本文选题：慢性阻塞性肺疾病 + 补肺益肾方　；参考：《辽宁中医杂志》2016年08期

【摘要】：目的:评价补肺益肾方联合舒肺贴对慢性阻塞性肺疾病(COPD)稳定期大鼠T淋巴细胞亚群的影响。方法:大鼠72只随机分为空白组、模型组、舒肺贴组、补肺益肾组、补肺益肾联合舒肺贴组(简称联合组)、氨茶碱组,12只/组。采用熏烟联合细菌感染法制备COPD稳定期大鼠模型。自第9周,各组分别以相应方案处理,第20周结束后取材。观察大鼠肺功能及外周血和支气管肺泡灌洗液(BALF)中CD_3~+、CD_4~+、CD_8~+、CD_4~+/CD_8~+的表达。结果:模型组外周血、BALF中CD_3~+、CD_4~+、CD_4~+/CD_8~+水平较空白组明显降低(P0.01),CD_8~+水平明显升高(P0.01)。与模型组相比,各治疗组外周血、BALF中CD_4~+、CD_4~+/CD_8~+水平升高(P0.05,P0.01),CD_8~+水平降低(P0.05,P0.01);联合组CD_3~+水平升高(P0.05,P0.01)。联合组外周血、BALF中CD_4~+、CD_4~+/CD_8~+较穴位贴敷组升高(P0.05,P0.01),CD_8~+下降(P0.05);BALF中CD_4~+、CD_4~+/CD_8~+较补肺益肾组升高(P0.05)。结论:补肺益肾联合舒肺贴可调节CD_3~+、CD_4~+、CD_4~+/CD_8~+表达,从而提高机体免疫功能,发挥治疗作用。
[Abstract]:Aim: to evaluate the effect of BFYS combined with Shufei paste on T lymphocyte subsets in stable rats with chronic obstructive pulmonary disease (COPD). Methods: 72 rats were randomly divided into blank group, model group, Shufei paste group, combined group of tonifying lung and kidney, aminophylline group (12 rats / group). The stable COPD rat model was established by fumigation combined with bacterial infection. From the 9th week, each group was treated with the corresponding scheme, and the materials were collected after the 20th week. The lung function and the expression of CD3 / CD4 / CD8 / CD8 ~ + in peripheral blood and bronchoalveolar lavage fluid (BALF) of rats were observed. Results: compared with the control group, the level of CD3 ~ / CD8 ~ in the BALF of the model group was significantly lower than that in the control group. Compared with the model group, the level of CD4 ~ / CD8 ~ in the peripheral blood BALF of each treatment group was increased, and the level of CD8 ~ in the combined group was decreased, and the level of CD3 ~ in the combined group was increased (P _ (0.05) P _ (0.01) and the level of CD8 ~ in the combined group was increased (P _ (0.05) P _ (0.01). CD4 / CD8 ~ + in BALF of the combined group was higher than that in the acupoint application group. The level of CD8 ~ decreased in P0.05 and BALF and CD4 ~ / CD8 ~ in the BALF was higher than that in the group of tonifying lung and tonifying the kidney. Conclusion: tonifying the lung and tonifying the kidney combined with Shufei plaster can regulate the expression of CD4 ~ / CD4 ~ / CD8 ~ + in CD3 / CD4 / CDT, thus improve the immune function of the body and play a therapeutic role.
【作者单位】：河南中医药大学第一附属医院中心实验室(中药呼吸药理实验室);河南中医药大学老年医学研究所;呼吸疾病诊疗与新药研发河南省协同创新中心;
【基金】：国家自然科学基金重点项目(81130062) 国家中医药管理局国家中医临床研究基地业务建设科研专项(JDZX2012030)
【分类号】：R563.9

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