骨髓间充质干细胞不同时相干预对SD大鼠急性肺损伤的修复作用

发布时间：2018-06-02 03:42

本文选题：骨髓间充质干细胞 + 急性肺损伤　；参考：《广东医学》2017年05期

【摘要】：目的探究骨髓间充质干细胞(BM-MSCs)不同时间干预对以静脉注射脂多糖(LPS)构建大鼠急性肺损伤(ALI)模型的修复作用。方法体外分离、培养并鉴定大鼠BM-MSCs。将120只雄性SD大鼠纳入研究,分成对照组、LPS组和LPS+BM-MSCs组,上述3组因BM-MSCs处理时间差异分成2、8、24、48、96 h共5个亚组(n=8)。LPS组以5 mg/kg尾静脉注射LPS,LPS+BM-MSCs组在注射LPS后分别在上述不同时间点尾静脉注射1 mL的BM-MSCs(1×10~6·mL~(-1));对照组则在上述时相点注射等剂量的生理盐水。检测各组肺泡灌洗液肿瘤坏死因子-α(TNF-α)、白细胞介素(IL)~(-1)α等炎症因子浓度、肺湿干比及肺损伤病理评分。结果成功分离、培养、纯化BM-MSCs,并诱导向成骨、成软骨、成脂细胞分化;流式细胞术鉴定所培养细胞系CD90、CD105、CD45、CD34阳性率分别为98.89%、97.37%、3.17%及1.41%。在LPS诱导的ALI中,TNF-α、IL~(-1)α、IL~(-1)0在8 h亚组中测得最高值[分别为(364.911±32.182)pg/mL、(286.316±18.567)pg/mL、(162.037±22.377)pg/mL],随着时间延长逐渐下降;肺湿干比在2 h亚组中测得最高值(6.361±0.265);肺水肿及组织损伤程度在2 h亚组中最严重,病理评分也测得最高值(0.462±0.048),随着时间延长逐渐下降。在LPS+BM-MSCs组中,2、8、24 h的炎症因子、湿干比及病理评分均有显著改变(P0.05),随着时间延长,差异无统计学意义(P0.05)。结论早期应用BM-MSCs可减少TNF-α、IL~(-1)α等炎症因子,从而减轻肺组织损伤及肺泡水肿。
[Abstract]:Objective to investigate the effects of BM-MSCs on the repair of acute lung injury (Ali) induced by intravenous lipopolysaccharide (LPS) in rats. Methods BM-MSCs were isolated, cultured and identified in vitro. 120 male SD rats were divided into control group and LPS BM-MSCs group. The above three groups were divided into 5 subgroups for 96 h because of the difference of BM-MSCs treatment time. The BM-MSCs group was injected with 5 mg/kg tail vein injection of LPS LPS-LPS-1L of 1 mL of BM-MSCs(1 脳 10 6 mL-1 at different time points after LPS injection, while the control group was injected at the above time point at the same time point. The same dose of saline. The concentrations of inflammatory factors such as tumor necrosis factor- 伪 (TNF- 伪) and interleukin-1 (IL-1a) in alveolar lavage fluid were measured. The wet / dry ratio of lung and pathological score of lung injury were also measured. Results BM-MSCs were successfully isolated, cultured, purified, and differentiated into osteogenic, chondrogenic and adipogenic cells, and the positive rates of CD90, CD105, CD45, CD45 and CD34 were 98.89, 97.37, 3.17% and 1.41%, respectively, by flow cytometry. In the ALI induced by LPS, the highest values of TNF- 伪 / L ~ (1) were measured in the 8 h subgroup [364.911 卤32.182 g / mLU 286.316 卤18.567 渭 g / mL ~ (-1)], and the lung wet / dry ratio in 2 h subgroup showed the highest value (6.361 卤0.265), pulmonary edema and tissue damage were the most serious in the subgroup of 2 h, respectively, and the degree of lung edema and tissue damage was the most serious in the subgroup of 2 h. The ratio of wet to dry of lung was 6.361 卤0.265 in the subgroup of 2 h, and the degree of lung edema and tissue damage was the most serious in the subgroup of 2 h. The ratio of wet to dry of lung was 6.361 卤0.265 in the subgroup of 2 h. The highest value of pathological score was 0.462 卤0.048, which decreased gradually with time. In the LPS BM-MSCs group, the inflammatory factors, wet dry ratio and pathological score were significantly changed at 824 h in the LPS BM-MSCs group, but there was no significant difference between them with the prolongation of time. Conclusion the early application of BM-MSCs can reduce the inflammatory factors such as TNF- 伪 and ILA) 伪, thus alleviate the lung tissue injury and alveolar edema.
【作者单位】：广东药科大学附属第一医院干保科;南方医科大学第五附属医院呼吸内科;
【基金】：广东省财政技术研究开发与推广应用专项基金项目(编号:粤财工[2013]401号)
【分类号】：R563

【相似文献】