大剂量辣椒素耗竭初级感觉传入神经对兔肺缺血再灌注损伤氧化应激的影响

发布时间：2018-06-04 09:49

本文选题：缺血再灌注 + 肺脏　；参考：《四川大学学报(医学版)》2014年02期

【摘要】：目的运用化学去神经法阻断辣椒素敏感的C类神经纤维(CapsCF),观察对肺缺血再灌注损伤(IR)的影响,并探讨氧化应激反应在其中的作用。方法新西兰大白兔32只随机分为4组:假手术组(S组)、肺缺血再灌注组(IR组)、辣椒素组(CS组)、辣椒素肺缺血再灌注组(CIR组)。分别予以辣椒素(100mg/kg)或其载液皮下注射,5d后建立肺IR模型。实验终末检测血气分析,氧化应激指标〔丙二醛(MDA)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)〕、肺组织湿干比,HE染色观察肺组织病理学改变。结果与S组和CS组比,IR组和CIR组动脉血氧分压降低(P0.05),肺泡动脉氧分压差升高(P0.05),肺组织湿干比升高,氧化应激反应加重(P均0.05),病理学损伤加重。CIR组指标改变均较IR组加重(血气学指标除外)。结论化学去神经法阻断CapsCF,加重肺IR损伤,其机制可能与加重氧化应激反应有关。
[Abstract]:Objective to investigate the effects of oxidative stress on capsaicin sensitive C nerve fibers capsCFN by chemical denervation in order to observe the effects of capsaicin C nerve fibers on lung ischemia-reperfusion injury (IRI) and to explore the role of oxidative stress in the blockage of capsaicin C nerve fibers. Methods Thirty-two New Zealand white rabbits were randomly divided into 4 groups: sham operation group (group S), pulmonary ischemia-reperfusion group (group IR), capsaicin group (group C), capsaicin group (group C) and capsaicin group (group C). Pulmonary IR model was established 5 days after administration of capsaicin 100 mg / kg or subcutaneous injection of capsaicin. Blood gas analysis, oxidative stress index (malondialdehyde MDAA), superoxide dismutase (SOD), catalase (catalase) and lung tissue wet / dry ratio (HE) staining were used to observe the pathological changes of lung tissue. Results compared with S group and CS group, the arterial partial pressure of oxygen in IR group and CIR group was lower than that in group S and CIR group, the difference of oxygen pressure in alveolar artery was increased (P0.05), and the wet / dry ratio of lung tissue was increased. The oxidative stress reaction was aggravated (P < 0.05), the pathological injury was aggravated. The changes of indexes in CIR group were more serious than those in IR group (except blood gas index). Conclusion Chemical denervation of Caps CFS may be related to increased oxidative stress.
【作者单位】：四川大学华西医院麻醉科;深圳南山医院麻醉科;
【基金】：国家自然科学基金(No.81170077)资助
【分类号】：R562

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